logos biosystems LUNA FX7 - GF;RF Exposure Level; Cell Size Calculation; Min.;Max. Cell Size; GF;RF Threshold Level

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GF exposure level
0.1-10
5
RF exposure level
0.1-10
5
Cell size calculation
BF/FL
BF
Min. cell size
1-89
3
Max. cell size
2-90
70
GF threshold level
1-10
5
RF threshold level
1-10
5
Dilution factor
1-10
1.11
The IQOQ-FL on the Protocol List is the protocol that is used for IQOQ with the fluorescence
validation slide.
! Important ! The value of total concentration printed on the validation slide label, using
the IQOQ-FL may differ from that of using the default protocol because the label value
is determined with the IQOQ-FL protocol, which is for the purpose of IQOQ and Quality
Control mode.
GF/RF Exposure
Level
The exposure for each channel can be adjusted. Increase exposure if the preview image is dim
and only a few cells are visible. Lower exposure if the preview image is too bright and
background noise is high. Determine optimal exposure values empirically.
Cell Size
Calculation
A mode between brightfield and fluorescence can be selected. The selected mode is used to
measure the cell size.
If FL is selected, the cell size is measured based on the fluorescence signals so the results may
change depending on the exposure level.
Min./Max. cell size
Use these parameters to adjust the minimum and maximum cell sizes to be included in results.
The base unit is 1 micrometer.
GF/RF threshold
level
Green and Red fluorescence threshold will
determine the level of threshold during the image
processing. Increasing the threshold will lead to fewer cells being detected by increasing the
background level that is subtracted. Conversely, decreasing the threshold will lead to more cells
being detected.
Dilution Factor
The default dilution factor is pre-set as 1.11 for the standard Acridine Orange and Propidium
iodide staining protocol, (e.g. 18 µL cells + 2 µL AO/PI.)
Assuming a final volume of 20 µL, the dilution factor may be adjusted according to the table
below:
Dilution
factor
1
1.11
1.18
1.25
1.44
1.66
2
2.5
3.33
5
10
Sample
volume
20 µL
18 µL
17 µL
16 µL
14 µL
12 µL
10 µL
8 µL
6 µL
4 µL
2 µL