logos biosystems LUNA FX7 - Min.;Max. Search Size; Cell Detection Sensitivity; Live Cell Sensitivity; Noise Reduction

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Min./Max. search
size
Search size refers to the approximate cell size that the algorithm recognizes as potential cell
objects. By adjusting Min. and Max. search size, objects sized within the setting value will be
listed as a potential cell candidate.
Clustered objects larger than the Max. search size will not be excluded from the search. Rather,
the algorithm will utilize morphological information to identify individual objects within the cluster
that are within the search size parameters. For the most accurate results, it is recommended to
set the Min./Max. search size window as narrow as possible to encompass the expected cell
size range.
Cell detection
sensitivity
Cell detection sensitivity refers to the sensitivity of object separation from the background. A
higher Cell detection sensitivity value will increase detection of signals from weakly stained cells
or smaller objects, but can also increase false positive calls.
Live cell
sensitivity
Live cells with intact cell membranes exclude Trypan blue, Erythrosin B and Methylene blue.
The dyes stain the cytoplasm of dead cells with compromised membranes. As a result, object
intensity of unstained live cells is brighter than the stained dead cells. A higher Live cell sensitivity
will decrease the intensity cutoff value and increase the number of live cells detected.
Live Cell Sensitivity is not available in the protocol of the Total cell counting mode.
Noise reduction
This option allows for the adjustment of background noise during counting. With more noise
reduction, the instrument will not detect weakly stained objects. With lower noise reduction, the
instrument can detect objects with fainter signals.
Dilution factor
The dilution factor is used to calculate cell concentrations accurately. The default dilution factor
is preset as 1 for Total cell counting and as 2 for Total cell & viability counting
(assuming a 1:1
ratio of stain to cell suspension).
This value can be modified according to the dilution of the original sample
in increments of 1
between 1-10 and, increments of 10 between 10 -
100. For users handling highly dense cell
cultures. For highly
dense cultures, serial dilutions and several counts with appropriately
adjusted dilution factors may be necessary.
Fluorescence Cell
Counting
Parameters – Cell
lines & Primary
cells mode
Parameters for [Fluorescence cell counting-Cell lines & primary cells]
eter
Range
DEFAULT*