logos biosystems LUNA FX7 - Bioprocess Feature; Bioprocess

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Min. FL intensity
Min FL intensity is used to set the minimum Green and Red fluorescence intensity values to
be detected as cells. Objects with fluorescence intensity lower than Min. FL intensity value
are excluded from counting. Increased Min. FL intensity value will remove objects with weak
fluorescence intensity. Conversely, decreased Min FL. Intensity value will detect more objects
with weak fluorescence intensity.
Min. roundness
Roundness is the measure of how closely the shape of a cell approaches mathematically
perfect circle. Roundness is expressed as a value between 1 and 0, Roundness = 1 for a
perfect circle and Roundness = 0 for a line segment. Higher value will lead to the counting of
rounder cells and excludes objects with less roundness. Lower values are suitable for
counting cells with irregular shapes.
Dilution Factor
The default dilution factor is pre-
set as 1.11 for the standard Acridine Orange and Propidium
iodide staining protocol, (e.g. 18 µL cells + 2 µL AO/PI.)
Assuming a final volume of 20 µL, the dilution factor may be adjusted according to the table
below:
Dilution
factor
1
1.11
1.18
1.25
1.44
1.66
2
2.5
3.33
5
10
Sample
volume
20 µL
18 µL
17 µL
16 µL
14 µL
12 µL
10 µL
8 µL
6 µL
4 µL
2 µL