Merck Spectroquant Prove 300 User Manual

Version 1.1 – 10/2015
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4 Operation – 4.3 Zero adjustment for preprogrammed methods
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4.3 Zero adjustment for preprogrammed methods
A zero adjustment must be performed for each cell type. The zero
adjustment is stored within the spectrophotometer separately for
each cell type. The period of validity of the zero adjustment can be
edited in the System settings. When a zero adjustment has already
been performed for the inserted cell type and the selected
method, the date of the most recent zero adjustment is displayed
in the info line.
When a zero adjustment is required, following screen will pop up:
1. Insert the zero cell according to cell type. Zero adjustment
starts automatically and a tick
1
appears in the status display
󹭯eldforthezeroadjustment.Inthecaseofamethodwhich
only measures the sample at a single wavelength the absorb-
ance of the zero value
2
is also displayed.
2. With a cell inserted, the zero adjustment can be repeated
manually by tapping on the Start zero button
3
.
3. Tapping on the OK button accepts the zero adjustment value
for the method.
4. The screen changes to show the concentration measurement
screen (see page 7).
5. The instrument is ready to start measuring.
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4.3.1 Notes on zero adjustment
Zero adjustment with round cells:
• Only use clean, scratch-free round cells and distilled water.
Theminimum󹭯llinglevelis20mm.Areadyzerocellis
contained within the scope of delivery of the spectrophoto-
meter
• Areadyzerocellcan,inprinciple,beusedforaninde󹭯nite
period of time. We recommend, however, that you regularly
check the zero cell for visible contamination and scratches and
re󹭯llorexchangeitifnecessary(atleastevery24months)
• Insert the round cell until it touches the bottom of the round
cell compartment
Zero adjustment with rectangular cells:
• With rectangular cells, zero adjustment must be carried out
using the same cell type (manufacturer and cell material [e.g.
optical glass, quartz glass, plastic]) as the one that will be used
for measurement. This is important because cells of different
manufacturers have different absorbance characteristics.
When changing the cell type, repeat the zero adjustment with
the new type
• Priortozeroadjustment,cleantherectangularcelland󹭯llit
withdistilledwater.Theminimum󹭯llinglevelis20mm
• Rectangular cells always have to be inserted in the cell com-
partment with the same orientation for measurement and zero
adjustment (e.g. cell inscription always on the left side)
• Insert the rectangular cell until it touches the bottom and left
edge of the holder. The opaque sides of the rectangular cell
must point to the front and rear
NOTE
The instrument provides a Quick Zero function. With this the zero
adjustment is performed for all wavelengths which are used with
all Spectroquant® test kits. This function can be activated via
“System (Instrument settings)" – “Quality (Setup 3)".

Merck Spectroquant Prove 300 Specifications

General

Brand	Merck
Model	Spectroquant Prove 300
Category	Measuring Instruments
Language	English