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Molecular Devices SpectraMax M2 User Manual

Molecular Devices SpectraMax M2
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A MULTI-DETECTION MICROPLATE READER WITH TWO-MODE CUVETTE PORT
SpectraMax
®
M2/M2
e
user guide

Questions and Answers:

Molecular Devices SpectraMax M2 Specifications

General IconGeneral
Wavelength Range200-1000 nm
Detection ModesAbsorbance, Fluorescence, Luminescence
Light SourceXenon flash lamp
Wavelength SelectionMonochromator
Microplate Compatibility6- to 384-well plates
SoftwareSoftMax Pro
Power Requirements100-240 VAC, 50/60 Hz
ShakingYes
Dynamic RangeUp to 4.0 OD

Summary

1. Description of SpectraMax M2/M2e

1.1. Introduction to SpectraMax M2/M2e

Overview of the SpectraMax M2/M2e as a monochromator-based microplate reader with dual detection.

1.2. Key Components

Identifies main instrument components: Control panel, Microplate drawer, Cuvette chamber, Back panel.

2. Principles of Operation

2.1. Absorbance, Optical Density, and Transmittance

Defines absorbance, optical density, transmittance, and their relationships.

2.4. PathCheck Technology

Explains the Beer-Lambert law and its relation to pathlength and absorbance correction.

2.5. Fluorescence Measurement

Introduces fluorescence, absorption, electronic state change, and emission of light.

2.8. Functional Description

Highlights SoftMax Pro software for instrument control and direct instrument functions.

3. Installation Guide

3.1. Unpacking the SpectraMax M2/M2e

Instructions for unpacking the instrument, retaining packing materials, and checking components.

3.2. Setting Up the Instrument

Steps for placing the instrument on a level surface and connecting cables.

3.3. Installing the Drawer Adapter

Procedure for installing the drawer adapter for top reading of 96/384-well microplates.

3.4. Removing the Drawer Adapter

Instructions for removing the drawer adapter when using specific plate types or bottom reading.

4. Operation Procedures

4.1. Cuvette Read: Quick Overview

Provides a quick guide to reading a cuvette, including power, temperature, and wavelength selection.

4.2. Microplate Read: Quick Overview

Offers a quick guide to reading a microplate, including power, temperature, and loading steps.

4.4. Reading the Cuvette

Procedure for inserting, referencing, and reading a cuvette.

4.5. Reading the Microplate

Instructions for loading a microplate, using SoftMax Pro, and removing the plate.

4.6. Optimizing Fluorescence Assays

Introduction to optimizing settings like wavelengths, cutoff filters, PMT voltage, and temperature.

5. Maintenance and Support

5.1. Technical Support

Contact info for Molecular Devices Technical Support and safety precautions for service.

5.2. Moving the SpectraMax M2/M2e

Step-by-step instructions for safely relocating the instrument, including weight warning.

5.4. Cleaning Procedures

Instructions for cleaning the exterior surfaces of the instrument, including safety precautions.

5.6. Changing the Fuses

Instructions for replacing blown fuses, including fuse types and ratings.

6. Troubleshooting Common Issues

6.1. Opening the Drawer Manually

Procedure for manually opening the microplate drawer in case of errors or power outages.

6.2. Error Codes and Probable Causes

Overview of error codes, fatal errors, and warning messages displayed by the instrument.

7. Instrument Specifications

7.1. SpectraMax M2 and M2e Performance Specifications

Details thermal and microplate specifications, and cuvette reading requirements.

Absorbance Photometric Performance

Lists absorbance photometric performance specs: wavelength, range, accuracy, drift.

Fluorescence Photometric Performance

Lists fluorescence photometric performance specs: detection limit, wavelength range.

Temperature Regulation

Covers temperature regulation specs: range, resolution, accuracy, and warm-up time.

A. Appendix

A.1. Cables for Connectivity

Recommendations for high-quality, double-shielded cables for computer connection.

A.2. Recommended Accessories

Lists various accessories for the SpectraMax M2/M2e, including validation plates and power cords.

A.3. Cuvettes for SpectraMax M2/M2e

Provides guidelines for cuvette use and lists tested cuvettes with their dimensions.

A.4. Wavelengths for Fluorescence and Luminescence

Guide to determining optimal excitation/emission wavelengths based on literature and scanning.

A.8. Glossary of Terms

Definitions of key terms used in the user guide, such as Absorbance, Automix, Fluorescence.

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