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NanoDrop ND-1000 User Manual

NanoDrop ND-1000
61 pages
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ND-1000 Spectrophotometer
V3.5 User’s Manual
NanoDrop Technologies, Inc.
3411 Silverside Road
Bancroft Building
Wilmington, DE 19810 USA
Voice: 302-479-7707
Fax: 302-792-7155
Email: info@nanodrop.com
www.nanodrop.com
NanoDrop is a registered trademark of NanoDrop Technologies, Inc. Other parties
trademarks are the property of their respective owners and should be treated as such.
Copyright © 2007 NanoDrop Technologies, Inc.
rev 7/2007

Table of Contents

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NanoDrop ND-1000 Specifications

General IconGeneral
BrandNanoDrop
ModelND-1000
CategoryMeasuring Instruments
LanguageEnglish

Summary

Initial Setup and Software Installation

Computer Requirements and Software Installation

Specifies PC requirements and provides a step-by-step guide for installing NanoDrop software.

General Operation Procedures

Sample Retention System Operation

Explains the NanoDrop's sample retention technology, basic use, and cleaning.

Sample Handling and Preparation Tips

Covers special cleaning, sample size, carryover prevention, homogeneity, and solvent effects.

Application Modules Overview

Description of the different application modules available in the NanoDrop software.

Common Module Functions

Module Startup and Measurement Initiation

Starting modules, initiating measurements (F1), and performing initial blanking (F3).

Nucleic Acids Analysis

Nucleic Acids Sample Volume and Concentration

Sample volume requirements and measurement concentration ranges for nucleic acids.

Nucleic Acids Purity Ratios

Interpretation of 260/280 and 260/230 ratios for nucleic acid purity.

MicroArray Analysis

Fluorescent Dye Selection and Sample Volume

Selecting fluorescent dyes and recommended sample volumes for MicroArray analysis.

MicroArray Concentration Range

Concentration ranges for fluorescent dye and nucleic acid measurements in MicroArray.

UV-VIS Spectrophotometry

UV-VIS Sample Volume and Concentration Range

Recommended sample volume and measurable absorbance range for UV-VIS.

Protein A280 Quantification

Protein A280 Sample Volume and Pedestal Reconditioning

Recommended sample volume and pedestal reconditioning for A280 protein assays.

Protein A280 Concentration Range and Screen Features

Concentration range and explanation of screen elements for Protein A280 quantification.

Proteins and Labels Analysis

Proteins & Labels Dye Selection and Sample Volume

Selecting fluorescent dyes and recommended sample volumes for Proteins & Labels.

Proteins & Labels Pedestal Reconditioning

Procedure for reconditioning measurement pedestals for protein and label assays.

Proteins & Labels Concentration Range and Screen Features

Concentration ranges and explanation of screen elements for Proteins & Labels analysis.

Protein BCA Assay Procedure

Protein BCA Sample Volume and Pedestal Reconditioning

Recommended sample volume and pedestal reconditioning for BCA protein assays.

Protein BCA Concentration Range and Kits

Concentration ranges for BCA assays and guidelines for using BCA kits.

Protein BCA Screen Features and Measurement

Explanation of BCA screen elements and procedure for making BCA measurements.

Protein Lowry Assay Procedure

Protein Lowry Screen Features and Measurement

Explanation of Lowry screen elements and procedure for making Lowry measurements.

Protein Bradford Assay Procedure

Protein Bradford Screen Features and Measurement

Explanation of Bradford screen elements and procedure for making Bradford measurements.

Archived Data and Data Viewer

Calibration Check Procedure

Calibration Check Procedure Steps

Step-by-step guide for performing the CF-1 calibration check.

Troubleshooting Common Errors

USB Connection Errors (USB2000, Connection Error)

Troubleshooting steps for USB connection issues and errors.

Intensity Check and Detector Saturation Errors

Performing an intensity check and resolving saturated detector errors.

Column Detection Warnings and Pedestal Reconditioning

Interpreting warnings about column formation and performing pedestal reconditioning.

Sample Accuracy and Blanking Cycle

Tips for improving sample accuracy and performing a blanking cycle.

Unusual Spectrum Diagnosis and Technical Service

Diagnosing unusual spectra and contacting technical service for support.

Maintenance and Warranty

Cleaning Procedures and Calibration

Routine cleaning of pedestals and information on calibration.