NEW BRUNSWICK SCIENTIFIC BioFlo 4500

Questions and Answers

J
John BrooksAug 1, 2025
Why my NEW BRUNSWICK SCIENTIFIC Laboratory Equipment fails to communicate with BioCommand?
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  mmillsAug 1, 2025
  There are several reasons why your equipment might fail to communicate with BioCommand: 1. The Multidrop (Unit ID) Address assigned to the Controller in BioCommand might be incorrect. Verify that a single Node Number (Unit ID or Multidrop Address) value of 0 through 19 has been assigned to the Controller. 2. There may be a conflicting Multidrop (Unit ID) Address with other equipment in BioCommand. Assign a new Unit ID to the equipment and verify that a single Node Number (Unit ID or Multidrop Address) value of 0 through 19 has been assigned to the Controller. 3. The Controller software configuration might have been deleted due to battery backup and power failure. Verify that the Controller configuration has been saved, and reload the configuration into the software.
E
Eric SmithAug 5, 2025
What to do if NEW BRUNSWICK SCIENTIFIC BioFlo 4500 Laboratory Equipment shows a message that Local Controller is not responding?
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  Wendy Johnson PhDAug 5, 2025
  If you receive a message that the Local Controller is not responding, there are several potential causes: * There may be no power to the fermentor console. Verify the power cord connections. * The External Computer Cable may not be connected properly. Check that the cable is connected and secured to the proper ports on both the computer and fermentor. * There may be an internal controller error. Contact your local NBS Service Department.
M
Michael GreerAug 9, 2025
How to fix no power indicator on NEW BRUNSWICK SCIENTIFIC BioFlo 4500 Laboratory Equipment fermentor console?
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  cathyortegaAug 9, 2025
  If there is no power indicator on the fermentor console, the following could be the problem: * There is no power to the fermentor console. Verify the power cord connections. * Verify power switch operation. If the issue persists, contact your local NBS Service Department.
C
cperezAug 13, 2025
Why is the touchscreen monitor blank on my NEW BRUNSWICK SCIENTIFIC BioFlo 4500?
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  Jennifer ReedAug 13, 2025
  A blank touchscreen monitor could be due to: * No power to the monitor. Verify the power cord connections to both the monitor and console. * No power to the computer. Verify that the LED POWER INDICATOR is illuminated on the computer.
X
xschroederAug 18, 2025
How to improve poor loop control on NEW BRUNSWICK SCIENTIFIC Laboratory Equipment?
- C
  catherine89Aug 18, 2025
  To improve poor loop control: * Confirm proper configuration settings and connections. * Recalibrate probes and sensors.
G
grantjustinAug 22, 2025
What causes inaccurate agitation loop readings in NEW BRUNSWICK SCIENTIFIC BioFlo 4500 Laboratory Equipment?
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  Matthew PerezAug 22, 2025
  Inaccurate agitation loop readings can be caused by: * Loose cable connections. Check cable connections. If all connections are firmly seated, replace the speed sensor.
J
Julie JimenezAug 27, 2025
What to do if NEW BRUNSWICK SCIENTIFIC BioFlo 4500 Laboratory Equipment displays an overflow error message?
- L
  Linda WrightAug 27, 2025
  If an overflow error message appears on the display, press OK to acknowledge the message. Then, press the START BUTTON, and select SHUTDOWN. In the SHUTDOWN WINDOWS DIALOG, select “Restart the Computer?” and press YES.

Summary

4 Getting Started

4.1 Positioning the Fermentor

4.2 External Computer Set-up

8 ML-6100 Controller Configuration

8.1 Configuring the Controller

Procedure for configuring the ML-6100 controller for various process parameters.

8.3 Control Mode Selection and Node Number

Setting the controller's instrument name, node number, and control mode.

10 ML-6100 Temperature Control System

11 ML-6100 Agitation Control System

11.1 Operation

14 ML-6100 Pump Control

14.1 Pump Capacity

14.4 Pump Calibration

Procedure for calibrating each pump using total or rate functions.

16 ML-6100 pH Control

16.1 Calibration of pH Probe

Steps for calibrating the pH probe, including liquid and gel-filled types.

28 ML-6100-Controlled Automatic Sterilization

28.1 Sterilization (with Liquid In Vessel)

Procedure for automatic sterilization with liquid present in the vessel.

28.5 Aborting Sterilization

Procedure for stopping or aborting the sterilization process at any time.

34 Preventive Maintenance

34.1 Cleaning

Instructions for cleaning the fermentor console, cables, and vessel after use.

34.6 Battery Replacement

35 Troubleshooting

35.1 Troubleshooting Guide

A guide to identifying and resolving common operational issues and error messages.

NEW BRUNSWICK SCIENTIFIC BioFlo 4500 Specifications

General

Volume (Up to)	20 L
Temperature Control	Yes
pH Control	Yes
DO Control	Yes
Agitation Speed	50 - 1200 rpm
Data Logging	Yes
Applications	Cell culture, microbial fermentation
Agitation	Mechanical
Vessel Material	Glass
Total Vessel Volume	20 L