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NEW BRUNSWICK SCIENTIFIC BioFlo 4500 User Manual

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BioFlo 4500
Benchtop
Fermentor/Bioreactor
MANUAL NO: M1254-0050
Revision H
September 27, 2002
NEW BRUNSWICK SCIENTIFIC CO., INC.
BOX 4005 44 TALMADGE ROAD EDISON, NJ 08818-4005
Telephone: 1-732-287-1200 1-800-631-5417
Fax: 732-287-4222 Telex: 4753012 NBSCO
Internet: http://www.nbsc.com E-mail: bioinfo@nbsc.com

Table of Contents

Questions and Answers:

NEW BRUNSWICK SCIENTIFIC BioFlo 4500 Specifications

General IconGeneral
Volume (Up to)20 L
Temperature ControlYes
pH ControlYes
DO ControlYes
Agitation Speed50 - 1200 rpm
Data LoggingYes
ApplicationsCell culture, microbial fermentation
AgitationMechanical
Vessel MaterialGlass
Total Vessel Volume20 L

Summary

4 Getting Started

8 ML-6100 Controller Configuration

8.1 Configuring the Controller

Procedure for configuring the ML-6100 controller for various process parameters.

8.3 Control Mode Selection and Node Number

Setting the controller's instrument name, node number, and control mode.

10 ML-6100 Temperature Control System

11 ML-6100 Agitation Control System

14 ML-6100 Pump Control

14.4 Pump Calibration

Procedure for calibrating each pump using total or rate functions.

16 ML-6100 pH Control

16.1 Calibration of pH Probe

Steps for calibrating the pH probe, including liquid and gel-filled types.

28 ML-6100-Controlled Automatic Sterilization

28.1 Sterilization (with Liquid In Vessel)

Procedure for automatic sterilization with liquid present in the vessel.

28.5 Aborting Sterilization

Procedure for stopping or aborting the sterilization process at any time.

34 Preventive Maintenance

34.1 Cleaning

Instructions for cleaning the fermentor console, cables, and vessel after use.

35 Troubleshooting

35.1 Troubleshooting Guide

A guide to identifying and resolving common operational issues and error messages.

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