NEW BRUNSWICK SCIENTIFIC BioFlo CelliGen 115 User Manual

154 pages

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New Brunswick Scientific PO Box 4005 44 Talmadge Rd. Edison, 08818-4005 USA

1.800.631.5417 1.732.287.1200 bioinfo@nbsc.com

www.nbsc.com

Guide to Operations

BioFlo

/CelliGen

115

Benchtop Fermentor & Bioreactor

MANUAL No: M1369-0050

Revision B

June 2, 2009

Default Chapter8
- Table of Contents8
1 Warnings & Cautions13
- Warnings13
- Cautions14
2 Inspection & Unpacking of Equipment16
- Inspection of Box(Es)16
- Packing List Verification16
- Basic Components16
3 Introduction & Overview17
- System17
- Vessels17
- Agitation System17
- Temperature Control18
- Aeration18
- Ph Control18
- Do Control19
- Foam/Level Control19
- Exhaust System19
- Recommended Accessories & Supplies19
- Supervisory Software20
4 Installation21
- Physical Location21
- Environment21
- Installing the Control Cabinet22
- Connecting Utility Cabinets24
- Utilities26
- Important Warnings29
- Vessel Assembly: Non-Jacketed31
- Vessel Assembly: Water-Jacketed38
- Main Power Switch56
- Optional Biocommand Software57
5 Specifications59
- Certifications60
6 Operating Controls62
- Touchscreen62
- Display Screens62
7 Probe Preparation & Calibration74
- Ph Probe Inspection74
- Ph Probe Calibration74
  - Ph Probe Installation75
  - Ph Probe Maintenance & Storage77
- Dissolved Oxygen (Do) Probe Preparation78
- Level Probe Calibration82
- About Pump Calibration83
8 Vessel Sterilization84
- Initial Preparation for Autoclaving85
- Autoclaving the Vessel86
  - Sterilization Time and Temperature87
9 Reinstalling the Vessel Assembly88
- Reinstall the Vessel Assembly88
- Load Pump Tubing88
- Confirm Ph Calibration90
- Install Liquid Addition Systems90
  - Addition Tubing Size91
- Reconnect Gases92
- Install Temperature (Rtd) Probe92
10 Cascade Control93
- Creating a Cascade94
11 About Pumps96
- Pump Assignment96
- Pump Setpoint97
- Pump Control Mode99
- Pump Flow Rate & Calibration Methods99
- Pump Period100
- Using Level Probes to Program Feed Pumps101
12 Using the Setup Screen104
- Controller Setup104
  - Gas Control106
- System Settings109
  - Resetting Date/Time109
  - Updating Software109
- Hardware Setup110
  - Identifying Utility Station(S) Added112
  - Removing a Utility Station112
13 Performing a Run113
- Set up Foam Control113
- Preparing for a Fermentation Run113
- Inoculation114
- Start Biocommand (if Present)115
- Sampling Procedure115
- Fermentation Phases115
- Batch Operation116
- Fed Batch Operation116
- Continuous Operation117
- Anaerobic and Microaerophilic Culture117
- Harvesting Procedure118
- Shutdown Procedure118
14 Essential Operating Tips120
- Precautions for Glass Vessel Assembly120
- Exhaust Condenser & Exhaust Filters120
- Install a Double Filter System121
15 Cleaning122
- Cleaning the Vessel122
  - List of Wetted Parts122
- Cleaning the Cabinet122
16 Maintenance123
- Ph Probe Maintenance and Storage123
- Do Probe Maintenance and Storage123
- Vessel & Tubing124
- Periodic Inspection124
- Agitator Bearing Housing124
  - Motor Assembly Replacement124
- Replacement Parts125
17 Service126
- Troubleshooting126
18 Drawings128
- List of Drawings128
- List of Tables129
19 Appendix A: some General Concepts130
- What Is a Controller130
- What Is a Control Loop130
- What Is Probe Calibration131
- What Are P-I-D Constants131
- What Is P-I-D Tuning132
- What Do the Constants Mean133
20 Appendix B: Otr134
- Determining an Oxygen Transfer Rate134
  - OTR Calculations134
- Some Factors that Affect Otr and Horsepower135
21 Appendix C: Fermentation Techniques137
- Media Formulation137
- Antifoam Formulation138
- Tubing Size138
- Acid & Base139
- Glucose Feed139
- Recommended Process Control Settings140
- Typical Fermentation Run140
22 Appendix D: Corrosion Resistance148
23 Appendix E: General Characteristics of Epr149
- Identifying Epr149
- General Characteristics149
24 Index150

Questions and Answers:

D
Dalton WoodAug 14, 2025
Why is there insufficient gas flow in my NEW BRUNSWICK SCIENTIFIC Laboratory Equipment?
- C
  Christopher AlvarezAug 14, 2025
  Insufficient gas flow can occur due to several reasons. The inlet or exhaust sterile air filter might be wet or clogged, in which case you should replace it. Ensure that the air pressure is within the specified range. Verify that the control mode for DO and pH is set to AUTO and that the GasFlo loop is ON. Also, confirm that the Air loop is in O2 Enrichment mode and that the DO cascades are Enabled.
E
ejordanAug 17, 2025
What to do if my NEW BRUNSWICK SCIENTIFIC BioFlo CelliGen 115 Laboratory Equipment is leaking water?
- M
  Manuel BrooksAug 17, 2025
  If your unit is leaking water, the inlet water pressure may be too high. Try lowering the pressure to stay within the recommended range. Also, check all inlet hose connections to ensure they are tight; tighten them if necessary.

NEW BRUNSWICK SCIENTIFIC BioFlo CelliGen 115 Specifications

General

Temperature Control	Yes
pH Control	Yes
DO Control	Yes
Power Requirements	100-240 VAC, 50/60 Hz
Type	Fermentor
pH Control Range	2 - 12
Dissolved Oxygen (DO) Control Range	0 - 100%
Control System	Digital
Material	Stainless steel or glass
Sterilization	Autoclavable