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Nikon TIRF User Manual

Nikon TIRF
37 pages
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M301 E 03.6.CF.2
TIRF SYSTEM FOR TE2000
< INSTRUCTIONS >
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Nikon TIRF Specifications

General IconGeneral
BrandNikon
ModelTIRF
CategoryDigital Camera
LanguageEnglish

Summary

Introduction and Safety Precautions

WARNING and CAUTION Symbols

Explains the meaning and significance of WARNING and CAUTION symbols used in the manual.

Meaning of a Symbol Used on the Equipment

Details the meaning of a specific symbol indicating laser danger on the equipment.

Safety Precautions

Laser Safety

Details laser safety standards and compliance for the system.

Eyepiece Shutter Usage

Emphasizes the mandatory use of the eyepiece shutter to prevent eye injury.

Laser Emission Position Warnings

Laser Emission Position

Identifies areas where laser beams are emitted and warns against looking directly at them.

General Safety Procedures

Items to Check Before Turning on the Laser

Lists essential checks to perform before activating the laser system.

Do Not Look Into the Laser Beam

Reiterates the danger of looking into the laser beam and avoiding reflective surfaces.

Component Handling Safety

Do Not Disassemble

Prohibits disassembling the system due to electric shock and laser hazards.

System Power and Environmental Safety

Power Supply Ratings and Cords

Details specifications for the AC adapter and power cords for safe operation.

Prevent Contact with Moisture

Precautions regarding moisture to prevent short circuits and malfunctions.

System Handling and Installation

Handling

Provides guidelines for careful handling of the precision optical instrument.

Installation Conditions

Specifies environmental and physical conditions for safe system installation.

Name and Function of Each Part

System Configuration Overview

Overview of the components that make up the TIRF system.

Optical Path Diagram (TIRF Microscopy)

Diagram illustrating the light path for TIRF microscopy operations.

TIRF Attachment

Description of the TIRF attachment's role and components.

Component Details

Optical Path Diagram (TIRF Microscopy)

Diagram illustrating the light path for TIRF microscopy operations.

TIRF Attachment Components

TIRF Attachment

Description of the TIRF attachment's role and components.

TIRF Attachment Controls

Laser Position Adjustment Knobs

Explanation of how to adjust the laser beam position for proper illumination.

Field Diaphragm Adjustment

How to adjust the field diaphragm for illumination area control.

TIRF Attachment Optics

Focal Point Adjustment and Lens Locking

How to adjust the focal point for optimal laser beam performance.

Specimen Cover Functionality

Specimen Cover

Details the specimen cover's filter function and usage.

TIRF Cassette Holder Operations

TIRF Cassette Holder

Explanation of the TIRF cassette holder and its function.

Shutter Lever Function

Function of the shutter lever for interrupting the laser beam.

Laser Unit Information

Laser Unit

Details about the laser unit(s) used in the system.

Laser Unit Components and Safety

ND Filter Slider for Intensity Adjustment

How to use the slider to change laser intensity using ND filters.

Critical Caution Label on Laser Unit

A critical caution label regarding removing covers and potential laser exposure.

Shutter Control Unit Overview

Shutter Control Unit

Information on the shutter control unit for managing laser shutters.

Power Switch and Lamp Indicators

How to turn the unit on/off and interpret power status indicators.

Eyepiece Shutter Connection

Connects the microscope-mounted eyepiece shutter.

Shutter Control Unit Features

Serial IF Connector for PC Control

Interface for PC control of the shutter system.

Timed Shutter Operation

How to program motorized shutters for timed operation.

Eyepiece Shutter Importance

Eyepiece Shutter Function

Details the function and importance of the eyepiece shutter for laser safety.

Getting Started with Operations

Getting Started (Emitting the Laser)

Initial steps to get the laser system ready for operation.

Laser Emission Procedures

Emitting the Argon Laser

Step-by-step guide to turn on and stabilize the argon laser.

Laser Operation

Emitting the He-Ne Laser

Guide to turning on and stabilizing the He-Ne laser.

Emitting the Solid-State Laser

Instructions for powering up the Solid-State laser.

TIRF Microscopy Operations

Operations (TIRF Microscopy)

Detailed steps for performing TIRF microscopy.

Achieving Focus with Epi-fl Microscopy

How to achieve focus using the epi-fl attachment before TIRF.

TIRF Microscopy and Shutdown

TIRF Microscopy Procedure

Detailed steps for performing TIRF microscopy.

Turning Off Power

Steps for safely powering down the entire system.

Simple Surface Reflection Interference

Simple Surface Reflection Interference Observations Using Epi-fl

Using Epi-fl for simple surface reflection interference observations.

Aperture and Field-Diaphragm Adjustment

How to adjust aperture and field diaphragm for optimal viewing.

Maintenance Procedures

Replacing the Halogen Lamp

Step-by-step guide for replacing the halogen lamp.

System Maintenance

Replacing Objectives and Filter Blocks

How to replace objectives and filter blocks.

Cleaning the Lens Section

Instructions for cleaning the lens section of optical components.

Shutter Control Unit Communications

Communication-Controlled Operation

How to control the system via PC using RS-232C interface.

Connector Specification

Specification of the D-sub 9-pin serial interface connector.

Communication Protocols

Communications Format

Defines the data format for commands and responses.

Command Format Details

Structure of commands sent to the system.

Response and Error Handling

Response Format

Structure of responses received from the system.

Error Codes for Troubleshooting

List and explanation of error codes for troubleshooting.

Shutter Control Commands

System Commands

Detailed list of commands for controlling shutter operations.

Troubleshooting Common Issues

Dark TIRF Image Solutions

Addresses problems causing dark TIRF images.

Poor TIRF Image Contrast Solutions

Provides solutions for TIRF images with poor contrast.

Performance Specifications

Applicable Lasers

Lists the types of lasers compatible with the system.

Product Safety and Compliance

Product Safety Certifications

Lists product safety certifications and compliance standards.

Laser Safety Classifications

Laser product safety classifications and standards.

FCC and Canadian EMI Regulations

FCC and Canadian EMI regulations compliance.

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