Nikon TIRF - Component Details; Optical Path Diagram (TIRF Microscopy)

I Name and Function of Each Part
2 Optical path diagram (TIRF microscopy)
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2
Optical path diagram (TIRF microscopy)
Optical path diagram
(1) Dichroic mirror
for combining
excitation light
(2) ND filter
(ND4, ND8)
(3) Motorized shutter
(4) Optical-fiber cable
(6) ND filter (ND2, ND8, ND32)
(7) ND filter (ND16)
(8) Condenser lens
(9) Dichroic mirror
Detach the excitation filter.
(10) Objective
(11) Specimen
(5) Collimator lens
(2) ND filter
(ND4, ND8)
(3) Motorized shutter
(1) Dichroic mirror
for combining
excitation light
Three-laser unit
Two-laser unit
TIRF attachment TIRF cassette holder
Laser from
either of
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