Nikon TIRF - Table of Contents

Content
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Content
Introduction...................................................................................................................................... 1
Safety Precautions (Read the following before use!)........................................................................ 1
I Name and Function of Each Part............................................................................................. 9
1 System configuration..................................................................................................... 9
2 Optical path diagram (TIRF microscopy) ..................................................................... 10
3 TIRF attachment ......................................................................................................... 11
4 TIRF cassette holder................................................................................................... 15
5 Laser unit .................................................................................................................... 16
6 Shutter control unit ...................................................................................................... 18
7 Eyepiece shutter ......................................................................................................... 20
II Operations............................................................................................................................. 21
1 Getting started (Emitting the laser).............................................................................. 22
2 Operations (TIRF microscopy) .................................................................................... 24
3 Turning off power ........................................................................................................ 25
4 Simple Surface Reflection Interference Observations Using Epi-fl Attachment ............ 26
4-1 Objectives ................................................................................................................ 26
4-2 Attachment Setup Procedure................................................................................... 26
III Maintenance.......................................................................................................................... 27
1 Replacing the halogen lamp........................................................................................ 27
2 Replacing the objective and the filter block.................................................................. 28
3 Moving or relocating the system.................................................................................. 28
4 Cleaning the lens section ............................................................................................ 28
IV Shutter Control Unit Communications Commands................................................................. 29
1 Communication-controlled operation ........................................................................... 29
1-1 Communications system.......................................................................................... 29
1-2 Connector specification ........................................................................................... 29
1-3 Cable specification................................................................................................... 30
1-4 Communications parameters................................................................................... 30
1-5 Communications format........................................................................................... 30
1-6 Communications sequence ..................................................................................... 31
1-7 Commands .............................................................................................................. 32
V Troubleshooting..................................................................................................................... 33
VI Performance and Specifications ............................................................................................ 34