Promega Quantus Fluorometer User Manual

Technical Manual
Quantus™ Fluorometer
Operating Manual
INSTRUCTIONS FOR USE OF PRODUCT E6150.
PRINTED IN USA.
Revised 1/20 Part# TM396
TM396.0120_EIVD_TM.qxd 1/27/2020 2:50 PM Page a

Promega Quantus Fluorometer Specifications

General

Detection Method	Fluorescence
Detector	Photodiode
Light Source	LED
Connectivity	USB
Excitation Source	LED
Emission Wavelength Range	485 nm, 560 nm, 670 nm

Summary

1. Introduction

1.C Specifications

1.D Precautions and Special Instructions

Outlines important safety warnings, handling precautions, and special usage instructions.

2. Quantus™ Fluorometer Inspection and Setup

2.A Setting Up the Quantus™ Fluorometer

2.B Setting Up the Quantus™ Software (Optional)

3. Operating the Quantus™ Fluorometer

3.A Calibrating the Quantus™ Fluorometer for Use With the QuantiFluor® Dye Systems

Step-by-step guide for calibrating the instrument with specific Promega dye systems.

3.C Calibrating the Quantus™ Fluorometer for Use With Other Dye Chemistries

Calibration process for user-defined protocols using different dye chemistries.

3.D Quantitating Samples

Detailed steps for measuring and quantifying sample concentrations using the instrument.

4. Cleaning and Maintenance

5. Troubleshooting

5.A Common Problems

Addresses common issues users might encounter and their potential causes.

5.B Error Messages

Explains various error codes and messages displayed by the instrument.