Promega Quantus Fluorometer - Page 10

2. Prepare the QuantiFluor
®
Dye working solution with 1X TE buffer as
follows. For example, to make a 1:400 dilution, combine 10µl of
QuantiFluor
®
Dye with 3,990µl of 1X TE buffer, and mix.
3. Prepare the nucleic acid standard in a 0.5ml PCR tube. Use the volume of
supplied standard and volume of 1X TE buffer indicated in the table below
to prepare the standard.
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Printed in USA. Part# TM396
Revised 1/20 Page 9
QuantiFluor
®
Dye System Dilution of QuantiFluor
®
Dye
ONE dsDNA (Cat.# E4871), single-
concentration standard curve
no dilution necessary
(dye is pre-diluted)
dsDNA (Cat.# E2670), single-
concentration standard curve
1:400
RNA (Cat.# E3310), high-
concentration standard curve
1:400
RNA (Cat.# E3310), low-
concentration standard curve
1:2,000
ssDNA (Cat.# E3190), high-
concentration standard curve
1:400
ssDNA (Cat.# E3190), low-
concentration standard curve
1:2,000
QuantiFluor
®
Dye System
Starting
Standard
Concentration
Volume of
Standard
Volume of
Working
Solution
Final
Standard
Calibration
(per tube)
ONE dsDNA (Cat.# E4871) 400ng/µl 1µl 200µl 400ng
dsDNA (Cat.# E2670) 100ng/µl 2µl 200µl 200ng
RNA (Cat.# E3310), high-
concentration standard curve
100ng/µl 5µl 200µl 500ng
RNA (Cat.# E3310), low-
concentration standard curve
100ng/µl
First dilute
1:100 in
1X TE
Buffer, then
use 10µl
200µl 10ng
ssDNA (Cat.# E3190), high-
concentration standard curve
100ng/µl 4µl 200µl 400ng
ssDNA (Cat.# E3190), low-
concentration standard curve
100ng/µl
First dilute
1:100 in
1X TE
Buffer, then
use 10µl
200µl 10ng
TM396.0120_EIVD_TM.qxd 1/27/2020 2:50 PM Page 9