SCIEX 6500 series User Manual

Name: SCIEX 6500 series
Brand: SCIEX
Rating: 4 (1 reviews)

181 pages

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6500 and 6500+ Series of Instruments

System User Guide

August 2015RUO-IDV-05-2095-A

Default Chapter3
- Table of Contents3
Chapter 1 Operational Precautions and Limitations8
- General Safety Information8
- Regulatory Compliance8
- Electrical Precautions10
  - AC Mains Supply10
  - Protective Earth Conductor11
- Chemical Precautions11
  - System Safe Fluids12
- Ventilation Precautions13
- Environmental Precautions14
  - Electromagnetic Environment15
  - Decommissioning and Disposal15
- Qualified Personnel16
- Equipment Use and Modification16
- Contact Us17
- Technical Support17
- Related Documentation17
Chapter 2 Hazard Symbols18
- Occupational Health and Safety Symbols18
- Symbols, Indicators, and Labels: Packaging20
- Symbols, Indicators, and Labels: Mass Spectrometer22
- Documentation Symbols and Conventions23
Chapter 3 Principles of Operation24
- System Overview24
Chapter 4 Operating Instructions Sample Workflows41
Chapter 5 Operating Instructions Hardware44
- Start up the System44
- Reset the Mass Spectrometer46
- Shut down the System46
- Plumb the Mass Spectrometer47
Chapter 6 Operating Instructions Hardware Profiles and Projects54
- Hardware Profiles54
- Projects and Subprojects60
Chapter 7 Operating Instructions Tune and Calibrate64
- Optimize the Mass Spectrometer64
- About the Verifying or Adjusting Performance Dialog65
- Results Summary65
Chapter 8 Operating Instructions Automatic Optimization67
- About Automatic Optimization67
  - Types of Sample Introduction68
- Automatically Optimize for an Analyte Using Infusion68
- Automatically Optimize for an Analyte Using FIA74
Chapter 9 Operating Instructions Acquisition Methods81
- Create an Acquisition Method Using the Acquisition Method Editor81
- Scan Techniques83
  - Quadrupole-Mode Scan Types83
  - LIT-Mode Scan Types84
- About Spectral Data Acquisition85
Chapter 10 Operating Instructions Batches86
- Set Queue Options86
- Add Sets and Samples to a Batch87
- Submit a Sample or Set of Samples90
- Change Sample Order91
- Acquire Data91
- Set Sample Locations in the Batch Editor91
- Select Vial Positions Using the Locations Tab (Optional)92
- Set Quantitation Details in the Batch Editor (Optional)93
- Stop Sample Acquisition94
- Import and Submit Batch Files94
  - Build a Batch as a Text File94
  - Import a Batch as a Text File95
- Batch Editor Right-Click Menu95
- Queue States and Device Status96
Chapter 11 Operating Instructions100
- Quantitative Analysis100
- Peak Review and Manual Integration of Peaks107
- Calibration Curves112
- Sample Statistics115
  - View the Statistics for Standards and Qcs115
- Compare Results between Batches116
Chapter 12 Operating Instructions Analyze and Process Data117
- Open Data Files117
- Navigate between Samples in a Data File117
- Show Experimental Conditions118
- Show Data in Tables119
- Show ADC Data121
- Show Basic Quantitative Data121
- Chromatograms122
- Show Tics from a Spectrum123
- Show a Spectrum from a TIC123
- About Generating Xics123
- Generate Bpcs126
- Generate Xwcs127
- Generate DAD Data127
- Generate Twcs128
- Adjust the Threshold128
- Chromatogram Panes129
- Spectra Panes130
- Data Processing130
- Graphs131
Chapter 13 Reporter Software134
- Reporter User Interface135
- Generate Reports137
Chapter 14 Service and Maintenance Information138
- Recommended Maintenance Schedule138
- Clean the Surfaces140
- Clean the Front-End140
- Empty the Source Exhaust Drain Bottle145
- Storage and Handling148
Chapter 15 Mass Spectrometer Troubleshooting149
Appendix A Operating Instructions Manual Optimization150
- About Manual Compound Optimization151
- About Scan Types151
- Manually Optimize an Analyte151
- Manually Optimize the Ion Source and Gas Parameters157
  - Prepare the Ion Source157
  - Optimize the Ion Source Parameters157
Appendix B 6500 and 6500+ Series System Parameters159
Appendix C Calibration Ions and Solutions163
Appendix D Toolbar Icons165
- Revision History173
- Index174

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Questions and Answers:

T
tiffanywashingtonJul 27, 2025
Why is the vacuum pressure too high and causing a system fault in my SCIEX 6500?
- A
  annagrayJul 27, 2025
  A system fault might occur in your SCIEX Laboratory Equipment due to high vacuum pressure because: * The roughing pump oil level is too low. Inspect the roughing pump oil level, and then contact the local QMP or an FSE to add oil. * There is a leak. Inspect and repair leaks. * The wrong orifice plate is installed. Install the correct orifice plate.

SCIEX 6500 series Specifications

General

Polarity Switching	Yes
Dynamic Range	> 5 orders of magnitude
Mass Analyzer	Triple Quadrupole
Ion Source	Turbo V Ion Source
Ionization Source	Electrospray Ionization (ESI) and Atmospheric Pressure Chemical Ionization (APCI)