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SCIEX X500 QTOF User Manual

SCIEX X500 QTOF
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SCIEX X500 QTOF System
System User Guide
August 2017RUO-IDV-05-2334-E

Table of Contents

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SCIEX X500 QTOF Specifications

General IconGeneral
BrandSCIEX
ModelX500 QTOF
CategoryLaboratory Equipment
LanguageEnglish

Summary

Chapter 1 Operational Precautions and Limitations

General Safety and Regulatory Compliance

Covers general safety precautions and regulatory compliance for system operation.

Electrical and Chemical Precautions

Details electrical safety, grounding, and chemical handling precautions.

Ventilation, Environmental, and Disposal Precautions

Discusses ventilation, environmental conditions, and decommissioning/disposal.

Chapter 2 Principles of Operation

System Overview and Hardware Components

Describes the system's components and provides a hardware overview with diagrams.

Quantitative Analysis and Calibration

Describes quantitative analysis, integration, results tables, and calibration curves.

Chapter 3 Operating Instructions—Hardware

System Start Up and Shut Down Procedures

Step-by-step instructions for starting up and safely shutting down the instrument.

Diverter Valve Operation and Plumbing

Explains the diverter valve's function and how to plumb it in different modes.

Chapter 4 Configure Devices

Device Management: Add, Edit, Delete, Deactivate

Procedures for managing system devices via the Configuration workspace.

Chapter 5 Configure Access to the Software

User Management: Adding and Deactivating Users

Procedures for managing user accounts, including adding and deactivating.

Chapter 6 Operating Instructions—Software

Software Navigation: Home Page, Workspaces, and Ribbon

Overview of the software's home page, workspaces, ribbon, and launcher for navigation.

Status Panel and Device Control

Explains the status panel functions and controlling device status in the software.

Project Management and Data Acquisition Panel

Covers adding/selecting projects and using the data acquisition panel for real-time analysis.

System Calibration Procedures: CDS and LC Methods

Procedures for calibrating the system using the CDS or an LC method.

System Equilibration and Queue Management

Instructions for system equilibration and managing the sample queue for acquisition.

MS Method Workspace

Creating and Configuring MS Methods

Step-by-step guide to creating new MS methods, including experiments and parameters.

Explorer Workspace: Analyte Verification

Verifying Analyte Presence and Extracting Ions

How to open samples, view chromatograms, and extract specific ions for analysis.

Analytics Workspace

Analytics Workspace Overview and Secure Export

Overview of the Analytics workspace, role-based access, and secure export settings.

Project Settings and Processing Method Creation

Defining project defaults and creating new processing methods for data analysis.

Creating and Editing Processing Methods

Detailed steps for creating and editing processing methods, including workflows and parameters.

Review Peaks

Using the Peak Review Pane for Data Inspection

Overview of the Peak Review pane for visually inspecting and correcting chromatogram integrations.

Analyzing Peaks with Library Search and Formula Finder Results

Using library search and formula finder results to analyze peaks and update processing.

Integration Algorithm Parameters

MQ4 Integration Parameters: Noise and Baseline

Details MQ4 integration parameters like noise percentage and baseline subtraction window.

Peak Splitting and AutoPeak Integration Parameters

Parameters for peak splitting and AutoPeak integration, including baseline and saturation correction.

MS Tune

Performing a Quick Status Check

Procedure to calibrate the system and verify resolution in TOF MS and MS/MS modes.

Optimizing Detector Sensitivity

Steps to adjust detector voltage for optimal sensitivity and save settings.

Tuning Q1 and TOF MS Parameters

Procedures for tuning Q1 and TOF MS parameters for optimal performance.

Tuning Q1 High Parameters

Adjusting Q1 parameters for high resolution and peak width optimization.

Advanced Troubleshooting and Restoring Instrument Data

Advanced troubleshooting steps and restoring previous instrument settings.

Chapter 7 Service and Maintenance Information

Recommended Maintenance Schedule and System Tasks

Provides a recommended schedule for cleaning and maintaining the system.

Mass Spectrometer and Ion Source Maintenance

Lists specific maintenance tasks for various mass spectrometer and ion source components.

Cleaning External Surfaces and Front-End Components

Procedures for cleaning external surfaces and front-end components of the mass spectrometer.

Cleaning the Front-End and Symptoms of Contamination

Instructions for cleaning the front-end and identifying symptoms of contamination.

Preparing the Mass Spectrometer for Maintenance

Steps to prepare the mass spectrometer before performing maintenance, including deactivating devices.

Cleaning the Curtain Plate

Detailed instructions for cleaning the curtain plate, including removal and aperture cleaning.

Cleaning the Orifice Plate and Reassembling the System

Procedures for cleaning the orifice plate and putting the mass spectrometer back into service.

Replacing the Check Valve and Flow Module

Instructions for replacing the check valve and flow module in the calibrant delivery system.

Move the Mass Spectrometer

Moving Procedures and Safety Precautions

Detailed steps and safety warnings for moving the mass spectrometer, including lifting requirements.

Chapter 8 Mass Spectrometer Troubleshooting

Basic System Issues and Troubleshooting Table

A table listing common system symptoms, possible causes, and corrective actions.

Troubleshooting: Sensitivity and Performance Degradation

Addresses issues related to reduced sensitivity and degraded mass spectrometer performance.

Troubleshooting: Arcing, Sparks, and Calibrant Signal Issues

Covers troubleshooting steps for arcing, sparks, and low calibrant signal problems.

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