Name: SCIEX CESI 8000 Plus
Brand: SCIEX
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CESI 8000 Plus High Performance

Separation-ESI Module

User Manual

RUO-IDV-05-3897-B

February 2019

Default Chapter3
- Table of Contents3
CHAPTER 1 Safety, Notices and Labels13
- Alerts for Warning, Caution, Important, and Note14
- Instrument Safety Precautions15
- Chemical Precautions17
- Safety Symbols and Labels18
- Rohs Notices19
  - China Rohs Caution Label19
- Other Instrument Labels20
- Overview13
安全、通知和标签23
- 综述23
- 警告、注意、重要事项以及注释的提示24
- 仪器安全防护措施25
- 化学品注意事项27
- 安全符号和标签27
- Rohs 通知28
  - 中国 Rohs 警告标签29
- 其他仪器标签29
CHAPITRE 3 Sécurité, Consignes Et Étiquettes31
- Alertes « Avertissement, Mise en Garde, Important Et Remarque32
- Précautions de Sécurité Pour L'instrument33
- Précautions Chimiques36
- Symboles Et Étiquettes de Sécurité36
- Autres Étiquettes de L'instrument38
- Consignes Rohs38
  - Étiquette de Mise en Garde Rohs (Chine)38
- About this Manual41
  - Optional Detectors41
- Présentation31
CHAPTER 4: System Overview41
System Overview41
- Introduction42
- Instrument43
- Sample Handling System44
- Syringe Pump, Power Supply, Leds and Interlock46
- Optims Cartridge48
CHAPTER 5 Integrating the CESI 8000 Plus System with a Mass Spectrometer51
- CESI 8000 Plus System: Installing Optims Cartridge53
  - Optims Cartridge Installation53
- Thermo Scientific Mass Spectrometer: Integration with the CESI 8000 Plus System57
- SCIEX Tripletof ® 5600 Mass Spectrometer: Integration with the CESI 800071
CHAPTER 6 32 Karat Software Overview87
- Overview of 32 Karat Software87
  - System Administration87
- Controller and Instrument Start up88
- Using the Direct Control Window to Load the Cartridge and Samples91
  - Tripletof ® 5600 Mass Spectrometer Using AAO93
- Methods and Sequences94
- Creating and Editing a Method95
- Instrument Setup Window95
  - Instrument Setup - Initial Conditions95
- Instrument Setup - Time Program97
- Saving a Method102
- Overview of Creating a Sequence103
  - Single Run and Sequence Runs103
- Programming a Sequence104
  - Sequence Table108
- Sequence Validation109
CHAPTER 7 CESI 8000 Software111
- Overview of the CESI 8000 Software111
- Launching the CESI 8000 Software112
- Users114
- Application Selection115
- Run Application115
- Samples and Vials118
- Run Acquisition131
  - SCIEX Tripletof ® 5600 Mass Spectrometer Using AAO133
  - Graph Options134
- Sequence Transfer135
  - Describe Sequence141
  - Sequence Verification144
- Sequence Transfer Overview135
- Sequence Display Options144
CHAPTER 8 Using a SCIEX Tripletof 5600 Mass Spectrometer with the CESI 8000 Plus System147
- Stock Reagents and Other Consumables Required148
- Preparation of Reagent Solutions and Test Sample149
- Procedure for Installation of Adapter and Optims Cartridge154
- Methods for the CESI 8000 Plus System155
  - CESI-MS Beta-Galactosidase System Performance Test159
- Capillary Conditioning162
- Establishing a Stable Spray and Determining Optimum ESI Voltage165
- Running CESI-MS172
- Introduction189
Using a Thermo Scientific Mass Spectrometer with the CESI 8000 Plus System189
- CESI-MS Integration Procedures51
- Introduction147
CHAPTER 9: Using a Thermo Scientific Mass Spectrometer with the CESI 8000 Plus System190
- Preparation of 10% (V/V) Acetic Acid (Hac)-Background Electrolyte (BGE)191
- Required190
- Sample191
- Cartridge194
- Capillary Conditioning195
  - Buffer Tray Setup195
  - Procedure196
- Voltage198
- Tuning202
- Running CESI-MS203
CHAPTER 10 Instruments217
- Shutdown217
- Shutdown and Decoupling Overview217
- Decoupling the CESI 8000 Plus System222
APPENDIX A: Specifications229
- CESI 8000 Plus System229
- Cart Specifications230
- Validated Controller Configuration230
- Capillary Temperature Control231
- Pressure and Vacuum System231
- Sample Temperature Control231
- Detector Specifications232
APPENDIX B: Nominal Network Configuration235
- Overview235
  - Systems236
- Switching from a SCIEX Mass Spectrometer to Another Mass237
  - Spectrometer237
- Switching to a SCIEX Mass Spectrometer from Another Mass239
  - Spectrometer239
- Advanced Networking and Computer Configuration for the Analyst ® TF241
APPENDIX C: Tripletof5600 Mass Spectrometer281
- Overview281
Tripletof ® 5600 Mass Spectrometer Manual and Auto-Calibration Methods281
- Methods for Auto-Calibration296
  - CE Method for Auto-Calibration296
  - MS Method for Auto-Calibration297
- Executing Auto-Calibration300
- Auto-Calibration Data Analysis312
  - Feature312
  - Automatic Rinse Feature312
- Failure317
  - Auto-Calibration Troubleshooting318
  - Table319
APPENDIX D: Auto Vial Increment Feature321
Feature321
- Overview321
- Step321
- Conditioning329
- Conductive Liquid Capillary329
Conductive Liquid Capillary (CLC) Conditioning329
- Procedure329
APPENDIX E: Conductive Liquid Capillary Conditioning , 329331
APPENDIX F: Using Vials and Hardware Maintenance331
Maintenance331
- Overview331
- Using CESI-MS Vials and Caps332
- Procedures334
- Storage)334
APPENDIX G: Troubleshooting349
- Diagrams349
- Overview349
- Procedure349
Troubleshooting349
- Fine Tuning Sprayer Tip Position for Thermo Scientific Mass Spectrometer364
- Fine Tuning Sprayer Tip Position for SCIEX Mass Spectrometer366
- CESI 8000 Plus Mobile Cart Homing Procedure368
- Contact Us369
- Customer Training369
- Cybersecurity369
- Online Learning Center369
- SCIEX Support369
- Documentation370
- Index371

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General

Brand	SCIEX
Model	CESI 8000 Plus
Category	Laboratory Equipment
Language	English

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