SCIEX CESI 8000 Plus User Manual

CESI 8000 Plus High Performance
Separation-ESI Module
User Manual
RUO-IDV-05-3897-B
February 2019

SCIEX CESI 8000 Plus Specifications

General

Brand	SCIEX
Model	CESI 8000 Plus
Category	Laboratory Equipment
Language	English

Summary

Safety, Notices and Labels

Alerts for Warning, Caution, Important, and Note

Defines the meaning and usage of Warning, Caution, Important, and Note alerts in the document.

Instrument Safety Precautions

Details essential safety measures to prevent operator injury during instrument operation and maintenance.

Moving Parts or Sharp Objects

Electrical Safety

Guidelines for preventing electrical injuries and property damage by inspecting electrical equipment.

Laser Safety (for Optional Laser Device)

Covers safety precautions related to the optional laser device, including its classification and hazards.

Chemical Precautions

Outlines necessary precautions for safe handling, use, and disposal of chemicals.

High Voltage Electric Shock Risk Symbol

Details the symbol indicating high voltage and the risk of electric shock.

Cancer and Reproductive Harm Label

System Overview

Instrument

Cartridge and Sample Cover Interlocks

OptiMS Cartridge

Describes the OptiMS cartridge assembly, including its capillaries, sprayer tip, and housing.

Integrating the CESI 8000 Plus System with a Mass Spectrometer

Physical Integration with the CESI 8000 Plus System

Provides steps for making physical connections between the CESI 8000 Plus system and a Thermo Scientific mass spectrometer.

OptiMS Cartridge Sprayer Tip Installation into a Thermo Scientific NanoSpray II Ion Source

Details the process of installing the OptiMS sprayer tip into a Thermo Scientific NanoSpray II ion source.

Establishing Communication Between the CESI 8000 Plus Controller and the SCIEX Mass Spectrometer

Explains how to establish communication between the CESI 8000 Plus controller and the SCIEX mass spectrometer.

Aligning the CESI 8000 Plus System with the SCIEX TripleTOF® 5600 Mass Spectrometer

Describes how to align the CESI 8000 Plus system with the SCIEX TripleTOF® 5600 mass spectrometer.

32 Karat Software Overview

System Administration

Details system administration features for controlling software access, security, and instrument settings.

License Key

Using the Direct Control Window to Load the Cartridge and Samples

Guides the user on how to use the Direct Control window to load cartridges and samples.

Methods and Sequences

Creating and Editing a Method

Provides instructions on how to create new methods or edit existing ones within the software.

Instrument Setup — Initial Conditions

Instrument Setup — Time Program

Saving a Method

Overview of Creating a Sequence

Programming a Sequence

Details the steps for creating a sequence to acquire data from multiple instrument runs.

Sequence Table

Sequence Validation

CESI 8000 Software

Login and Logout

Run Application

Enables routine users to run 32 Karat software methods and sequences, configure sample setup, and initiate runs.

Samples and Vials

Run Acquisition

Displays information about running sequences or methods and provides controls for monitoring and finishing runs.

How to Stop a Run

Sequence Transfer Overview

Describe Sequence

Explains how to describe sequence rows to define controls, samples, or always-run methods for routine users.

Using a SCIEX TripleTOF® 5600 Mass Spectrometer with the CESI 8000 Plus System

Methods for the CESI 8000 Plus System

Create a Sequence in the Analyst® TF Software

Step-by-step instructions for building a sequence in Analyst® TF software for performance testing.

Load Sample and Reagents on the CESI 8000 Plus System

Guides on loading samples and reagents onto the CESI 8000 Plus system based on sample tray setup.

Analyze Beta-Galactosidase Results

Using a Thermo Scientific Mass Spectrometer with the CESI 8000 Plus System

Stock Reagents and Other Consumables Required

Preparation of Reagent Solutions and Test Sample

Provides detailed instructions for preparing reagent solutions, including acetic acid and buffer solutions.

Procedure for Installation of Adapter and OptiMS Cartridge

Refers to Chapter 5 for adapter and OptiMS cartridge installation, and provides pre-installation alignment advice.

Capillary Conditioning

Describes the capillary conditioning procedure for OptiMS cartridges, including rinse steps.

Establishing a Stable Spray and Determining Optimum ESI Voltage

Guides on optimizing sprayer tip position and determining the optimum ESI voltage for stable spray.

Tuning

Covers the process of tuning the mass spectrometer, including creating tune files for CESI-MS operation.

Running CESI-MS

Provides a tutorial on running CESI-MS with a Thermo Scientific mass spectrometer using Xcalibur software.

Create a Method with the Xcalibur Software

Details how to create a method with two segments in Xcalibur software for CESI-MS analysis.

Create a Sequence with the Xcalibur Software

Guides on creating a sequence in Xcalibur software for queuing and running methods.

Start a Sequence with the Xcalibur Software

Provides steps to start a sequence in Xcalibur software, including setting instrument standby mode.

Load Sample and Reagents on the CESI 8000 System

Guides on transferring solutions and volumes into CESI-MS vials for the method.

Create a Sequence in the CESI 8000 Software

Details how to create a sequence in CESI 8000 software using a method with specific initial conditions.

Start a Sequence in the CESI 8000 Software

Instructions on how to run a sequence using the CESI 8000 software and initiate the run.

Analyze beta-galactosidase results

Shutdown Procedures and Decoupling Instruments

Cartridge Removal and the CESI 8000 Plus System Shutdown

Details the procedure for removing the OptiMS cartridge and shutting down the CESI 8000 Plus system.

Cartridge Removal and Instrument Shutdown

Decoupling the CESI 8000 Plus System

Decoupling from the Thermo Scientific Mass Spectrometer

Provides instructions for disconnecting the CESI 8000 Plus system from a Thermo Scientific mass spectrometer.

Decoupling from the SCIEX Mass Spectrometer

Provides instructions for disconnecting the CESI 8000 Plus system from a SCIEX mass spectrometer.

Nominal Network Configuration

Switching from the CESI 8000 Plus System to Different Mass Spectrometer Systems

Switching to the CESI 8000 Plus System from Different Mass Spectrometer Systems

Switching from a SCIEX Mass Spectrometer to Another Mass Spectrometer

Switching to a SCIEX Mass Spectrometer from Another Mass Spectrometer

Configuring Network Settings on the Analyst® TF Mass Spectrometer Acquisition Computer

Setting Analyst® TF Mass Spectrometer Acquisition Computer Firewall Parameters

Configuring Network Settings on the CESI 8000 Plus Controller

Setting the Mass Spectrometer Type

Curtain Gas Patch Installation

Details the installation process for a patch related to Curtain Gas settings for Beckman CE.

CE-MS Driver Installation

Provides instructions for loading and installing the BCI CE-MS interface driver.

Analyst Hardware Profile Configuration

Guides on configuring hardware profiles in Analyst TF software to recognize the CE instrument.

Conductive Liquid Capillary Conditioning

Conductive Liquid Capillary (CLC) Conditioning Procedure

Using Vials and Hardware Maintenance

Hardware Maintenance Procedures

Covers storage, removal/replacement of components like electrodes, levers, interface block, and fuses.

Capillary Cleaning Procedure (Short Term Storage)

Details the method for cleaning capillaries for short-term storage (up to 3 days).

Capillary Storage Procedure

Removing and Re-installing OptiMS Interface Plate

Provides steps for removing and re-installing the OptiMS interface plate for cleaning purposes.

Opening Levers, Electrodes, and the Interface Block

Guides on removing and replacing opening levers and electrodes, and accessing the interface block.

Clean Electrodes, Opening Levers, and the Interface Block

Details the procedure for cleaning electrodes, opening levers, and the interface block using water and methanol.

Refilling Coolant

Replacing Quad Rings

Replacing Fuses

Troubleshooting and Mobile Cart Homing Procedure

Troubleshooting and Flow Diagrams

Lists potential problems and provides corresponding corrective actions or flow diagrams for troubleshooting.

Blocked Sprayer Tip

Describes a problem where the sprayer tip is clogged and provides a procedure to resolve it.

Electrospray Cannot Be Established Between the CESI 8000 Plus System and Mass Spectrometer

Provides a flowchart to diagnose and troubleshoot issues preventing electrospray establishment between the CESI 8000 Plus and MS.

Electrospray is Detected at Zero Ion Spray (IS) Voltage while Applying CE Voltage

Unstable Electrospray while Applying CE Voltage and IS Voltage

Provides a flowchart to troubleshoot unstable electrospray conditions when applying CE and IS voltage.

Droplet at Capillary Tip Disappeared Indicating that Electrospray has Started, but No Mass Spectrometer Signal is Detected

Signal of Total Ion Current (TIC) is Lower Than Usual and/or Background Signal Intensity Fluctuates More Than 40%

Unstable CE Current

No Analyte Signals Detected by Mass Spectrometer During a CE-MS Separation

Provides a troubleshooting flowchart for cases where no analyte signals are detected by the mass spectrometer.

Some Analyte Peaks are Missing or Smaller Than Expected

CE Current Gradually Decreases During CE-MS Separation

The Iron-Acetate Cluster m/z 537 is Suppressing the Sample Signals

High Voltage System Unable to Deliver the Required Current

Provides a troubleshooting flowchart for issues where the high voltage system cannot deliver the required current.

Fine Tuning Sprayer Tip Position for Thermo Scientific Mass Spectrometer

Guides on optimizing sprayer tip position relative to the mass spectrometer inlet for proper ESI voltage.

Fine Tuning Sprayer Tip Position for SCIEX Mass Spectrometer

Details optimizing sprayer tip position relative to the curtain gas plate for proper ESI voltage with SCIEX MS.