Using a Thermo Scientific Mass Spectrometer with the CESI 8000 Plus System
CESI 8000 Plus High Performance Separation-ESI Module
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User Manual
RUO-IDV-05-3897-B | B11949AF
Before you start, ensure you have all the required reagents properly prepared. Description and
preparation protocols can be found in the next section.
Stock Reagents and Other Consumables Required
Table 9.1 Stock Reagents
Stock Reagent Vendor Part Number
Acetic Acid, Glacial Sigma-Aldrich A6283 or equivalent
0.1 N Sodium Hydroxide (NaOH) SCIEX 338424
0.1 N Hydrochloric Acid (HCl) EMD Chemical HX0603A
1 N Sodium Hydroxide (NaOH) Sigma-Fluka 319511
Methanol (MeOH) Fisher Chemical A454
7.5 M Ammonium Acetate (AmAc) Sigma-Aldrich A2706
Beta-Galactosidase Digest SCIEX 4333606
DDI water N/A 18 megaohm or higher
Table 9.2 Materials
Consumables Vendor Part Number
OptiMS silica surface cartridge SCIEX B07367
20 mL glass vial VWR VW74511-20
Micro vials SCIEX 144709
CESI-MS vial SCIEX B11648
Green CESI-MS vial cap SCIEX B24699
0.5 mL centrifuge tube
50 mL volumetric flask
20 mL glass bottle
100 mL glass bottle
pH meter
Table top mini centrifuge
Assorted pipettes and tips
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