Troubleshooting and Mobile Cart Homing Procedure
CESI 8000 Plus High Performance Separation-ESI Module
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User Manual
RUO-IDV-05-3897-B | B11949AF
Fine Tuning Sprayer Tip Position for SCIEX Mass Spectrometer
Before performing a CESI-MS separation, it is critical that the OptiMS sprayer tip position in
front of the curtain gas plate is optimized for proper ESI voltage. The sprayer tip should be
positioned outside the curtain gas plate to avoid rinsing solutions dripping into the curtain gas
plate. Having the sprayer too far from the curtain gas plate may require the use of high ESI
voltage which may lead to undesired peptide fragmentation. Figure G.5 depicts in green the
sprayer tip distance and ESI voltage values recommended when using a SCIEX mass
spectrometer. The red area should be avoided.
Figure G.5 SCIEX Mass Spectrometer Sprayer Tip Distance and ESI Voltage Correlation
Perform the following steps to optimize the sprayer tip position after placing OptiMS cartridge
on the CESI 8000 Plus system, the OptiMS sprayer on mass spectrometer adapter, and before
performing a CESI-MS separation:
Repeat this procedure:
1
Fill both OptiMS separation capillary and CLC with background electrolyte.
2
Align sprayer tip at 3 mm from curtain gas plate by adjusting xyz-knobs.
3
Apply CESI separation voltage (for example, 20 kV) that will be used in CESI-MS separation.
4
Ensure that CESI electrical current is stable.
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