52 | Page
MAINTENANCE
5. The CO
2
concentration in the incubation chamber must be stable in order to perform an accurate
calibration.
• The incubator must run for at least 8 hours prior to conducting a calibration.
• The CO
2
concentration is considered stabilized when the incubator has operated with the
door closed at your calibration CO
2
concentration for at least 1 hour with no fluctuations
greater than the specified stability of the unit (see page 56).
Continued next page
A Suggested CO
2
Calibration
1
Once the incubator CO
2
concentration has stabilized, compare the
reference device and the CO
2
display readings.
• If the readings are the same, or the difference
between the two falls within the acceptable range of
your protocol, the display is accurately showing the
incubation chamber CO
2
concentration. The CO
2
Calibration procedure is now complete.
-OR-
• If the difference falls outside of your protocol range,
advance to Step 2.
Reference Device
Set CO
2
%
2
A display calibration adjustment must be entered to match the
display to the reference device. See the next step.
Reference Device
X
(Exaggerated)
8 Hours Elapsed
(Minimum)