FUNCTIONAL DESCRIPTION
Sysmex KX-21 Operator’s Manual -- Revised October 1998 9-13
2 . WBC Histogram Error Flag
When the WBC histogram is normal with three peaks, there are a lower trough (T1)
and a higher trough (T2) between LOWER discriminator (LD) and UPPER
discriminator (UD).
• Output Example of Normal WBC Three-Peak Histogram
300
WBC
UD
200100
LD T1 T2
[fL]
WBC
LYM%
MXD%
NEUT%
LYM#
MXD#
NEUT#
6.7
28.3
17.4
54.3
1.9
1.2
3.6
[×10
3
/µL]
[%]
[%]
[%]
[×10
3
/µL]
[×10
3
/µL]
[×10
3
/µL]
Figure 9-6-2: Normal WBC Three-Peak Histogram
When TROUGH Discriminator (T1) or (T2) cannot be set or when frequency for a set
discriminator position is higher than the range, it is flagged as WBC histogram error.
Those histogram error flags are listed below in the order of higher priority. If more
than one flag are applied, the flag of the highest priority is taken.
WL: Relative frequency for LOWER discriminator (LD) exceeds the range. Probable
cause is inclusion of numerous platelet agglutinations, large platelets, and etc.
T1: Lower TROUGH Discriminator, that distinguishes lymphocytes and mixed cells,
cannot be not determined.
T2: Higher TROUGH Discriminator, that distinguishes mixed cells and neutrophils,
cannot be not determined.
F1: Small cell histogram error. Relative frequency for T1 exceeds the range.
F2: Middle cell histogram error. Relative frequency for T1 or T2 exceeds the range.
F3: Large cell histogram error. Relative frequency for T2 exceeds the range.
WU: Relative frequency for UPPER discriminator (UD) exceeds the range.
Applicable cases are those in which hemolysis is insufficient (for instance,
samples whose RBC membrane has extremely strong resistance against lyse) or
in which numerous abnormal blood cells are present.
AG: The particle count equal to or less than the LD exceeds a prescribed range.
Probable cause is platelet agglutination, which does not alter WBC count but
may result in decreased platelet count. Therefore, this flag is added to the PLT
parameter.
Sysmex KX-21 Operator’s Manual -- Revised December 1999
To Next Page
Loading...
