Thermo Scientific ISQ EM

Other manuals for Thermo Scientific ISQ EM

Preinstallation Manual56 pages

Questions and Answers

J
Jessica PaulJul 25, 2025
What to do if autotune fails after running for a while on Thermo Scientific Laboratory Equipment?
- A
  adamsmichaelJul 25, 2025
  If the Thermo Scientific Laboratory Equipment system fails autotune with a message indicating a spray stability check failure, examine the liquid connections for any leaks. Also, ensure that the electrospray needle is correctly adjusted. Unstable flow rates from the liquid pump can also cause this; AXP pumps need high back pressures for stable flow, so install backpressure coils between the pump and the instrument.
J
James TrevinoJul 31, 2025
Why does autotune fail at the beginning on Thermo Scientific Laboratory Equipment?
- L
  Lance RogersJul 31, 2025
  Autotune may fail at the beginning if the chromatograph is not set to 50 µL/min. Without sufficient liquid flow from the chromatograph, the calibrant won't be pushed into the ion source, causing the autotune to fail.
H
Hannah JacksonAug 4, 2025
What to do if the reference solution does not stop flowing after fill time in Thermo Scientific Laboratory Equipment?
- D
  Daniel RussellAug 4, 2025
  If the reference solution continues to flow after the fill time in your Thermo Scientific Laboratory Equipment, it might be due to the bottle not depressurizing because the frit on the valve of the calibrant delivery system is clogged. This valve is located on the gas control module under the main power supply inside the instrument. Alternatively, the waste line may be inserted too far down the drain tube, causing a siphoning effect.
K
Kelly WalkerAug 9, 2025
What to do if reference solution is not flowing down the drain during fill time in Thermo Scientific Laboratory Equipment?
- K
  Krista ReidAug 9, 2025
  If the reference solution isn't flowing down the drain or is flowing too slowly during fill time on your Thermo Scientific Laboratory Equipment, check the clear plastic pressurization line, green PEEK, or calibrant loop for kinks. Also, verify that the ferrules on the green PEEK tubing are not overtightened.
A
Aaron BaileyAug 14, 2025
What to do if reference solution is not flowing down the drain during fill time on Thermo Scientific Laboratory Equipment?
- L
  louismooreAug 14, 2025
  If the reference solution isn't flowing down the drain during fill time for your Thermo Scientific Laboratory Equipment, try replacing the calibrant bottle. Ensure the tubing's position is near the bottom of the bottle, but not touching it, to avoid picking up particulates that could cause clogging. Also, confirm that there is nitrogen supply to the instrument.
C
cwilcoxAug 19, 2025
How to fix leaky source gases in Thermo Scientific Laboratory Equipment?
- B
  Brandon BradfordAug 19, 2025
  If you're experiencing leaky source gases with your Thermo Scientific Laboratory Equipment, try tightening the spray chamber pins.

Summary

Safety

Safety Symbols and Signal Words

Details safety symbols, signal words, and their meanings in the manual and on the instrument.

Intended Use

Specifies the instrument's intended applications and limitations.

Safety Precautions

Provides essential safety information for installation, operation, and maintenance.

Solvent and Additive Information

Instrument Overview

Mass Spectrometer Operating Principle

Software Operation

Unpacking

Installation

Safety Guidelines for Installation

Outlines critical safety precautions and guidelines for installing the instrument.

Installing the Mass Spectrometer

Details the steps for installing and setting up the mass spectrometer hardware and software.

Site Requirements

Specifies environmental and physical requirements for the installation site.

Setting Up the Hardware

Provides instructions for setting up various hardware components, including pumps and filters.

Summary of Hardware Installation

Powering Up the System

Operation

Safety Guidelines for Operation

Provides crucial safety guidelines for operating the mass spectrometer.

Configuring the MS in Chromeleon

Details the process of configuring the mass spectrometer within Chromeleon software.

User Operation

Running Your First Sample

Shutting Down the MS Instrument

Maintenance and Service

Safety Guidelines for Maintenance and Service

Highlights safety precautions essential for performing maintenance and service.

General Rules for Maintenance and Service

Routine and Preventive Maintenance

Cleaning or Decontaminating the Instrument

Replacing the ESI Probe Capillaries for the ISQ EC and ISQ EM

Step-by-step instructions for replacing ESI probe capillaries.

Replacing the APCI Probe Capillaries for the ISQ EM

Instructions for replacing APCI probe capillaries on the ISQ EM.

Replacing the APCI Needle

Cleaning the Front Optics

Removing the Instrument from the System Stack

Updating the Mass Spectrometer Firmware

Troubleshooting

General Information about Troubleshooting

Troubleshooting Problems with Autotune

Provides a guide to diagnose and solve common autotune issues.

Troubleshooting Leaky Source Gases

Specifications

Physical Specifications

Thermo Scientific ISQ EM Specifications

General

Ion Source	Electron Ionization (EI)
Resolution	Unit Mass Resolution
Data Acquisition Rate	Up to 12, 500 u/sec
Software	Chromeleon CDS
Inlet	Compatible with HPLC and UHPLC
Vacuum System	Turbomolecular pump
Power Requirements	100-240 VAC, 50/60 Hz