Thermo Scientific NanoDrop 1000 V3.7 User Manual

Name: Thermo Scientific NanoDrop 1000 V3.7
Brand: Thermo Scientific
Rating: 5 (1 reviews)

105 pages

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NanoDrop 1000 Spectrophotometer

V3.7 User’s Manual

Default Chapter3
- Table of Contents3
1 Overview6
- Instrument Description6
- Operation6
- Applications6
- Patents7
2 Initial Set up8
- Computer Requirements8
- Software Installation8
- Software Upgrades9
- Registering Your Instrument10
3 General Operation11
- The Sample Retention System11
- Cleaning the Sample Retention System12
- Software Architecture and Features14
- User Preferences16
- Utilities and Diagnostics17
- Calibration Check17
- Account Management18
- Dye/Chromophore Editor20
4 Common Module Functions22
- Module Startup22
- Common Functions22
- Measure (F1)22
- Blank (F3)22
- Re-Blank (F2)23
- Print Screen (F4)24
- Start Report / Recording24
- Print Report (F5)25
- Show Report (F7)25
- Sample ID25
- Sample26
- Exit26
- Escape Key (ESC)26
- Show Context Help (Ctrl+H)26
- User's Manual26
5 Nucleic Acids27
- Sample Volume Requirements27
- Measurement Concentration Range27
- Spectrum Normalization29
- Spectrum Overlay Control29
6 Microarray31
- Fluorescent Dye Selection31
- Sample Volume Requirements31
- Measurement Concentration Range32
- Baseline Calculation & Normalization32
7 Uv-Vis35
- Sample Volume Requirements35
- Measurement Concentration Range35
- Unique Screen Features35
8 Protein A28037
- Sample Volume Requirements37
- Pedestal Reconditioning37
- Measurement Concentration Range37
- Unique Screen Features38
- Spectrum Normalization39
- Spectrum Overlay Control40
9 Proteins & Labels42
- Fluorescent Dye Selection42
- Sample Volume Requirements42
- Pedestal Reconditioning43
- Measurement Concentration Range43
- Unique Screen Features43
- Baseline Type45
10 Protein BCA46
- Sample Volume Requirements46
- Pedestal Reconditioning46
- Measurement Concentration Range46
- BCA Kits, Protocols, and Sample Preparation47
- Unique Screen Features47
- Making BCA Measurements48
- Standard Curve Features50
- Delete Standard Points50
- Exiting the BCA Module51
11 Protein Lowry52
- Sample Volume Requirements52
- Pedestal Reconditioning52
- Measurement Concentration Range52
- Modified Lowry Kits, Protocols, and Sample Preparation53
- Unique Screen Features53
- Making Lowry Measurements54
- Standard Curve Features56
- Delete Standard Points56
- Exiting the Lowry Module57
12 Protein Bradford58
- Sample Volume Requirement58
- Pedestal Reconditioning58
- Measurement Concentration Range59
- Bradford Kits, Protocols, and Sample Preparation59
- Unique Screen Features60
- Making Bradford Protein Measurements60
- Standard Curve Features62
- Delete Standard Points63
- Exiting the Bradford Module64
13 Protein Pierce 660 Nm65
- Unique Screen Features65
- Making Pierce 660 Nm Protein Measurements66
- Standard Curve Features68
- Delete Standard Points69
14 Cell Cultures70
- Sample Size Requirements71
- Cell Suspension Concentrations71
- Sample Homogeneity71
- Decontamination of Measurement Pedestals71
15 Archived Data and Data Viewer73
- Archive File Creation73
- Data Storage Hierarchy74
- Data Viewer74
- Archive File Converter81
- Opening Archived Data with Spreadsheet Programs82
16 Calibration Check83
- Procedure83
17 Troubleshooting86
- Error USB200086
- Connection Error87
- Signal Error89
- Saturated Detector90
- Liquid Column Breakage91
- Other Software Error Messages93
- Sample Accuracy and Reproducibility95
- 260/280 Ratio96
- Unusual Spectrum98
- Technical Support98
18 Maintenance and Warranty100
- Cleaning100
- Calibration100
- Warranty101
19 Appendices102
- Instrument Specifications102
- Blanking and Absorbance Calculations102
- Concentration Calculation (Beer's Law)103
- Solvent Compatibility104
- Decontamination of Measurement & Optical Surfaces104
- Setting up a Dymo 400 Label Writer Printer104

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Thermo Scientific NanoDrop 1000 V3.7 Specifications

General

Protein Concentration Range (A280)	0.1 - 100 mg/mL
Connectivity	USB
Sample Volume	1-2 µL
Concentration Range (dsDNA)	2-3700 ng/µL
Absorbance Precision	0.002 A (at 0.76 A)
Measurement Time	<5 seconds
Display	LCD
Power Supply	100-240 VAC
Path Length	0.2 mm (automatically adjusted)