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General| Wavelength Range | 190-850 nm |
|---|---|
| Sample Volume | 0.5 - 2 µL |
| Type | UV-Vis Spectrophotometer |
| Pathlength | 1 mm |
| Light Source | Xenon flash lamp |
| Display | 7-inch Touchscreen |
| Connectivity | USB |
| Absorbance Accuracy | 1% |
| Absorbance Precision | ± 0.002 A |
| Absorbance Linearity | 0 - 300 AU (1 cm equivalent) |
| Detection Limit | 2 ng/µL dsDNA |
| Maximum Concentration | 15, 000 ng/µL dsDNA |
| Protein Concentration Range (A280) | 0.1 mg/mL (BSA) |
| Measurement Time | <5 seconds |
| Operating Voltage | 100 - 240 V |
| Software | NanoDrop Software |
| Operating Temperature | 15-35°C |
| Operating Humidity | 20 - 80 % |
| Detector | 2048-element linear silicon CCD array |
Describes the two NanoDrop One models and their key components and differences.
Provides lower and upper detection limits and reproducibility for standard sample types.
Procedure to quantify purified double-stranded or single-stranded DNA or RNA samples.
Procedure to quantify nucleic acids labeled with up to two fluorescent dyes for microarray applications.
Procedure to quantify DNA or RNA samples using a user-defined extinction coefficient or factor.
Procedure to quantify oligonucleotides using user-defined base sequence for molar extinction coefficients.
Procedure to quantify proteins based on absorbance at 280 nm, using tryptophan/tyrosine content.
Procedure to quantify proteins and fluorescent dyes for labeled protein analysis and conjugation evaluation.
Procedure to quantify peptides and proteins based on absorbance at 205 nm, detecting peptide bonds.
Procedure for determining total protein concentration using a colorimetric assay with protein binding material.
Procedure to monitor microbial cell culture growth rate by measuring optical density at 600 nm.
Procedure to run user-defined measurement methods created using NanoDrop One Viewer software.
Procedure for using the instrument as a conventional spectrophotometer, measuring absorbance across UV and visible regions.
Provides instructions for connecting power, accessories, and setting up connections.
Step-by-step guide for performing micro-volume sample measurements on the instrument.
Step-by-step guide for performing measurements using the cuvette holder on NanoDrop OneC models.
Outlines daily, periodic, and 6-month maintenance tasks for the instrument.
Procedures for cleaning and reconditioning the instrument pedestals to ensure measurement integrity.
Information on running periodic performance and quality checks for instrument verification.
Explains safety symbols and special notices used in the user documentation.
Details precautions to avoid damaging the NanoDrop spectrophotometer during use.
Provides contact information for technical support in the U.S. and Canada.
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