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Thermo Scientific Orion AquaMate 8000 UV-Vis User Manual

Thermo Scientific Orion AquaMate 8000 UV-Vis
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269-289800 Revision A October 2012
UV-Vis
Orion AquaMate 7000 Vis
Orion AquaMate 8000 UV-Vis
User Guide

Table of Contents

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Thermo Scientific Orion AquaMate 8000 UV-Vis Specifications

General IconGeneral
BrandThermo Scientific
ModelOrion AquaMate 8000 UV-Vis
CategoryMeasuring Instruments
LanguageEnglish

Summary

Preface

Safety and Special Notices

Details on safety and special notices including notes, important information, cautions, and warnings.

Spectrophotometer Basics

Spectrophotometer Components

Describes the major external components of the spectrophotometer like the sample compartment and keypad.

Connectors

Details the location and types of connectors on the back of the instrument.

About the Keypad

Explains the function of each key on the instrument's keypad, including function keys and navigation keys.

Cell Holders

Describes the types of cell holders included and available for the Orion AquaMate spectrophotometers.

6-Position Cell Holder

Details the 6-position cell holder and its use with cuvettes.

Single Cell Holder

Describes the single cell holder and its use, including notes on its initialization.

Selecting and Positioning Cuvettes

Provides guidance on selecting cuvettes based on material and compatible wavelength ranges.

Z-dimensions

Explains the Z-dimensions and beam size specifications of the instrument.

Setting Up the Instrument

Entering Parameter Values

Instructions on how to input parameter values using the keypad, including numeric and alphanumeric entry.

Menu Selection

Guide on navigating menus and selecting options using the keypad for instrument setup.

On/Off Toggle

Explains how to toggle settings between ON and OFF states.

Alphanumeric Entry

Details the process of entering text strings for parameters like test names.

Setting Utility Parameters

Covers setting non-test hardware parameters like date, time, and screen contrast.

Setting the Date and Time

Step-by-step guide to setting the instrument's date, time format, and time.

Standby Mode

Information on the standby mode settings to prolong lamp life and its factory default.

Setting Baseline Expiration Time

Explains how to set a time limit for the validity of a collected baseline.

Setting the Screen Contrast

Instructions on adjusting the screen contrast for better readability.

Accessories

Cell Holders and Cell Holder Accessories

Describes sampling and system accessories, focusing on cell holders and their configurations.

Cell Holder Configurations

Presents a table of available cell holders and their accessories for instrument customization.

Cell Holder Initialization

Details the process of initializing the cell changer or cell holder upon starting a measurement.

Changing Cell Holders

Provides reasons and instructions for changing cell holders for different applications.

Installing the 6-Position Cell Holder and the Single Cell Holder

Step-by-step guide for installing the 6-position and single cell holders.

Removing the 6-Position Cell Holder and the Single Cell Holder

Instructions on how to properly remove the installed cell holders from the instrument.

Installing Accessory Cell Holders

Guidance on installing other accessory cell holders, referencing baseplates and securing mechanisms.

External Printers

Information on connecting and using external printers with the spectrophotometer.

Sample Positioner Setting

Auto 6

Describes the Auto 6 measurement option for measuring multiple samples sequentially.

Auto 3

Explains the Auto 3 measurement option for measuring a blank and two samples.

Manual 3

Details the Manual 3 setting specific to the Water Analysis test.

Single Cell Holder

Describes the manual measurement process using a single cell holder.

Manual 6

Instructions for manual measurements using the cell changer with up to five samples.

Cell Correction

Cell Correction

Explains how to perform cell correction to account for variances between cuvettes.

Setup Correction

Steps to set up the correction mode, including Scan and Discrete nms options.

Specifying Wavelengths for Discrete nms Mode

Guide on specifying wavelengths and the number of matched cuvettes for discrete nms mode.

Managing Stored Tests

Software Password

Information about the password feature to lock or unlock test setups.

Naming a Test

Instructions for assigning a unique alphanumeric name to a test.

Saving a Test

Explains the options for saving test methods to internal memory or an external device.

Loading Test Files

Guide on how to load saved test files from internal memory via the Utility screen.

Lock/Unlock

Procedure to lock or unlock stored tests using a password.

Deleting a Test

Instructions on how to delete a stored test from the instrument.

SmartStart

To set up a single test SmartStart

Steps to configure a single test to load automatically upon instrument power-up.

To unselect a test

Procedure to remove a test from the SmartStart menu.

To set up a multiple test SmartStart

Guide for setting up multiple tests to appear in the SmartStart menu.

Concentration Units

Specifying Concentration Units

Details the basic concentration units available for results labeling.

Creating Custom Units

Instructions on creating and adding custom concentration units to the list.

Calculator Function

To use the Calculator function

Step-by-step guide on using the instrument's built-in calculator for basic arithmetic.

Water Analysis Application

Loading a Test Method from the USB Stick to the AquaMate

Procedure for copying and loading water analysis test methods from a USB stick.

Running a Test Method from the USB Stick

Steps to run a water analysis test method that has been loaded from a USB drive.

Running a Test Method from the AquaMate Instrument Library

Guide to running water analysis tests stored in the instrument's library.

Running a Test Method from the AquaMate SmartStart Menu

Instructions for running water analysis tests selected from the SmartStart menu.

Running a Water Test

Details on executing a loaded water analysis test, including blank and sample measurements.

Programming a New Method or Altering the Factor(s) in a Method

Guide on creating new methods or modifying existing factors in water analysis tests.

Using the One Point Adjustment Calibration Feature

Explains how to use one-point adjustment for accurate measurements with preprogrammed methods.

Using a Reverse Color Method

Describes reverse color methods where concentration increases with decreasing absorbance.

Using a Preprogrammed Reverse Color Method

Steps to run a preprogrammed reverse color method, including blank and reagent blank measurements.

Programming a Reverse Color Method

Guide on programming a new reverse color test, including setting factors.

Abs and %T Measurements—Basic A-%T-C

Setting the Wavelength

Instructions for setting the desired wavelength for absorbance or %T measurements.

Measuring a Blank

Procedure for measuring a blank sample to establish a baseline for measurements.

Measuring Samples

Steps for measuring sample absorbance or %T values.

Abs and %T Measurements—Advanced A-%T-C

Recalling a Test

Procedure to recall a previously saved test in advanced mode.

Setting Up Test Parameters

Guide for configuring test parameters for advanced Abs/ %T measurements.

Taking Measurements

Steps for taking measurements in advanced Abs/ %T mode, with or without a cell changer.

Scanning

Collecting a Baseline Scan

Procedure for collecting a baseline scan for wavelength scanning.

Scanning a Sample

Instructions for scanning a sample to obtain its absorption or transmission spectrum.

Viewing and Manipulating Scan Data

How to view and manipulate scan results in graphical or tabular format.

Rescaling Graphical Scan Data

Procedure for rescaling graphical scan data using auto or manual scales.

Performing Calculations on the Scan Data

Details on performing calculations like labeling peaks and smoothing data.

Labeling Peaks and Valleys

Instructions for labeling peaks and valleys on a scan graph.

Smoothing Data

Guide on using the smoothing function to reduce noise in scan data.

Determining Peak Height Using a 3-point Net Equation

Procedure to determine peak height using a 3-point net equation.

Calculating the Area Under a Curve

Instructions for calculating the area under a peak in the scan graph.

Viewing and Rescaling Tabular Scan Data

How to view and rescale scan data presented in a tabular format.

Multiwavelength

Setting Up Test Parameters

Configuring parameters for multiwavelength measurements.

Adding Wavelengths and Factors

Procedure for adding specific wavelengths and their corresponding factors for measurement.

Deleting Wavelengths and Factors

Instructions on removing wavelengths and factors from a multiwavelength test.

Taking Measurements

Steps to perform multiwavelength measurements automatically.

Absorbance Ratio

Measuring Samples

Steps to measure samples for absorbance ratio analysis.

Absorbance Difference

Measuring Samples

Instructions for measuring samples for absorbance difference analysis.

Using the Absorbance Ratio Screen

Details on using the Absorbance Ratio screen for various test functions.

3-Point Net

Taking Measurements

Procedure to take measurements using the 3-Point Net application.

Concentration Measurements—Standard Curve Application

Creating a standard curve

Guide to setting parameters and measuring standards to create a calibration curve.

Measuring Samples

Steps for measuring samples using a previously created standard curve.

Editing a Standard Curve

Instructions on modifying a standard curve by changing data points or curve fit.

Setting the Parameters for a Standard Curve

Details on setting parameters for measuring standards for a calibration curve.

Measuring the Standards for a Standard Curve

Procedure for measuring standards to build a standard curve.

Using the Standards Screen

Explains how to use the Standards screen to view results, graphs, and edit curves.

Kinetics

Measuring Samples

Steps for measuring samples in kinetics analysis.

Recalling and Recalculating Graphical Kinetics Results

How to view and recalculate graphical kinetics data.

Rescaling and Recalculating Tabular Kinetics Results

Procedure for rescaling and recalculating tabular kinetics data.

Performance Verification

Accessing the Performance Verification Tests

Steps to access the instrument's performance verification tests.

Troubleshooting Checklist

Provides a checklist to diagnose common problems with performance verification tests.

Wavelength Accuracy - Internal

Tests the instrument's wavelength accuracy using internal standards.

Wavelength Accuracy - Standard

Measures wavelength accuracy against known standards.

Wavelength Repeatability (UV-Vis only)

Tests the instrument's ability to return to identical wavelengths repeatably.

Resolution (UV-Vis only)

Measures the instrument's ability to resolve adjacent spectral features.

Photometric Accuracy

Tests absorbance or %T against standards to check accuracy.

Noise

Measures the amount of noise in the instrument's readings.

Stray Light

Tests for stray light at selected wavelengths and compares with expected values.

Maintenance

Routine Care

Guidelines for basic routine maintenance to ensure instrument longevity and performance.

Cleaning and Maintaining Cells

Detailed instructions for cleaning cuvettes and other cells based on sample type.

Cleaning the Windows of the Sample Compartment

Procedure for cleaning the sample compartment windows without damage.

Changing the Fuse

Instructions for replacing the instrument's fuse, including fuse ratings.

Replacing the Tungsten Lamp

Step-by-step guide for safely replacing the tungsten lamp.

Parameters

Parameters

Explanation of various parameters used in instrument operations and tests.

Calculations for Software

Standard Curves

Formulas and methods for calculating standard curves, including partial sums.

Linear regression (general case)

Explains linear regression calculation for absorbance vs. concentration.

Linear regression through zero

Details the calculation of linear regression when the line passes through the origin.

Segmented model

Describes the segmented model for standard curve fitting.

Validity of standard curves

Criteria for validating standard curves, including ascending or descending slopes.

Statistics (Linear regression general case)

Formulas for calculating statistics like standard deviation and correlation coefficient.

Absorbance ratio

Formula for calculating absorbance ratio between two wavelengths.

Absorbance Difference

Formula for calculating the difference in absorbance between two wavelengths.

3-Point Net

Formula for calculating baseline corrected absorbance using a 3-point net.

3-Point Net (ASTM E169-04)

Formula for baseline corrected absorbance as per ASTM E169-04 standard.

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