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Thermo Scientific UltiMate 3000 - User Manual

Thermo Scientific UltiMate 3000
103 pages
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UltiMate™3000
RSLCnano
Standard Applications Guide
Revision: 3.1
Date: April 2019
Document No.: 4820.4103
Copyright © 2019 Thermo Fisher Scientific Inc. All rights reserved.
thermoscientific

Table of Contents

Question and Answer IconNeed help?

Do you have a question about the Thermo Scientific UltiMate 3000 and is the answer not in the manual?

Summary

Using this Manual

About this Manual

Describes the setups, recommended experimental conditions, and testing procedures for the system.

Conventions

Explains the standard notices, informational notes, and typographical conventions used throughout the manual.

Reference Documentation

Lists further information and resources related to the systems and associated applications.

Application Setup

General Recommendations for Applications

Provides general guidelines, schematics, installation tips, examples, and results for applications.

Available Trapping Columns

Details the two formats of trapping columns available: cartridge-based µ-precolumns and nano trap columns.

Installing the UltiMate 3000 RSLCnano System

Covers the components of the system, pump configurations, and software compatibility.

Direct Injection onto a Nano Column

Details hardware layout, fluidic setup, installation tips, and testing conditions for direct injection.

Pre-concentration onto a Nano Column

Describes hardware, fluidics, installation tips, and testing for pre-concentration setups.

Tandem Nano LC

Explains the setup and fluidics for tandem nano LC experiments, including testing.

MS Connection Kit

Lists common tubing, unions, and connectors for coupling the LC column to a mass spectrometer.

FAQs

NC_Pump Solvent Recalibration - Best Practice

Details best practices and frequency for solvent recalibration of ProFlow and classic flow meters.

Troubleshooting Nano LC Peptide Applications

Provides a decision tree and tips for diagnosing and resolving issues in nano LC peptide analysis.

Minimizing Baseline Effects

Offers strategies to reduce baseline drift and noise for optimal UV detection in LC applications.

Appendix

Customized Sample Injection Routines

Explains User Defined Programs (UDP) for creating custom injection routines and considerations.

Common Application Related Consumables

Lists common analytical columns and other consumables associated with the applications.

Summary

Using this Manual

About this Manual

Describes the setups, recommended experimental conditions, and testing procedures for the system.

Conventions

Explains the standard notices, informational notes, and typographical conventions used throughout the manual.

Reference Documentation

Lists further information and resources related to the systems and associated applications.

Application Setup

General Recommendations for Applications

Provides general guidelines, schematics, installation tips, examples, and results for applications.

Available Trapping Columns

Details the two formats of trapping columns available: cartridge-based µ-precolumns and nano trap columns.

Installing the UltiMate 3000 RSLCnano System

Covers the components of the system, pump configurations, and software compatibility.

Direct Injection onto a Nano Column

Details hardware layout, fluidic setup, installation tips, and testing conditions for direct injection.

Pre-concentration onto a Nano Column

Describes hardware, fluidics, installation tips, and testing for pre-concentration setups.

Tandem Nano LC

Explains the setup and fluidics for tandem nano LC experiments, including testing.

MS Connection Kit

Lists common tubing, unions, and connectors for coupling the LC column to a mass spectrometer.

FAQs

NC_Pump Solvent Recalibration - Best Practice

Details best practices and frequency for solvent recalibration of ProFlow and classic flow meters.

Troubleshooting Nano LC Peptide Applications

Provides a decision tree and tips for diagnosing and resolving issues in nano LC peptide analysis.

Minimizing Baseline Effects

Offers strategies to reduce baseline drift and noise for optimal UV detection in LC applications.

Appendix

Customized Sample Injection Routines

Explains User Defined Programs (UDP) for creating custom injection routines and considerations.

Common Application Related Consumables

Lists common analytical columns and other consumables associated with the applications.

Thermo Scientific UltiMate 3000 Specifications

General IconGeneral
Flow Rate Range0.001 to 10 mL/min
BrandThermo Scientific
Gradient MixingHigh-pressure or low-pressure mixing
Autosampler Temperature Range4 to 40 °C
Wavelength Range (UV-Vis)190 to 800 nm
Data Sampling RateUp to 100 Hz
SoftwareChromeleon Chromatography Data System
CategoryHPLC System
Pressure RangeUp to 1000 bar
Detection OptionsUV-Vis, Fluorescence, Refractive Index, Mass Spectrometry
System TypeModular
Pump TypeBinary, Quaternary
Injection Volume Range0.1 to 100 µL

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