What to do if Thermo Scientific Laboratory Equipment displays 'Flow meter recently changed'?
If the Zero Balance Test and the CalibrateWP command have not been performed after installing the ProFlow flow meter: 1. Perform the Zero Balance Test. 2. From Chromeleon, perform the CalibrateWP command for both pump heads.
Why Thermo Scientific Laboratory Equipment cannot regulate flow?
If the NC pump cannot build up sufficient pressure to deliver the selected flow rate, consider these potential causes and solutions: * Ensure the solvent reservoir is full. * Purge the pump to remove any trapped air. * Check for leaks in the system; find and eliminate any sources of leakage, tightening connections as needed. * Replace the frit in the solvent supply line filter if it's clogged. * Replace the inlet valve cartridge if it is blocked.
What to do if Thermo Scientific Dionex UltiMate 3000 cannot pressurize the system?
If pressure buildup fails, the possible causes and solutions are: * Check for air bubbles in the system and purge the pump. * If the inlet valve is defective, replace the valve cartridge. * If the solvent reservoir is empty, fill it and then purge the pump.
Why is there high noise level, non- periodic baseline fluctuation in Thermo Scientific Dionex UltiMate 3000 Laboratory Equipment?
A high noise level with non-periodic baseline fluctuation can be due to degraded or poor quality/purity eluent. Use fresh solvents, and ensure they are HPLC grade (MS grade if the SRD is operated with an NCS-3500RS or NCP-3200RS).
Why Thermo Scientific Security Sensors analysis run takes long time?
If your Thermo Scientific Security Sensors analysis run takes a long time to start, check the set flow cell temperature. If the flow cell can't reach the desired temperature at high flow rates, select a lower temperature.
What to do if Thermo Scientific Laboratory Equipment displays 'Zero position not found during the last 3 revolutions'?
Turn the module off and on again by pressing the power switch on the rear of the module.
What to do if Thermo Scientific Dionex UltiMate 3000 displays 'Flow meter missing'?
If communication with the flow meter failed, verify that the flow meter is installed properly.
What to check if Thermo Scientific Security Sensors have problems during control under Chromeleon?
If you encounter problems during control under Chromeleon with Thermo Scientific Security Sensors, check the USB cable and connection to the computer, as well as the USB port on the computer, ensuring it complies with the USB 1.1 standard.
What causes poor degassing in Thermo Scientific Laboratory Equipment?
Poor degassing can result from leaks in the capillaries or solvent supply lines, or if the flow rate is too high. Inspect the capillaries and solvent supply lines for leakage, tighten loose fitting connections, and reduce the flow rate.
Why flow calibration failed with Implausible measurement results in Thermo Scientific Dionex UltiMate 3000 Laboratory Equipment?
If flow calibration of the ProFlow flow meter yields implausible measurement results, a mismatch between the solvent and the sensor selected for calibration was detected. Repeat the flow calibration with the correct solvent or change the solvent. Contact Service.
General| Type | HPLC System |
|---|---|
| Data Sampling Rate | up to 100 Hz |
| Pump Type | Quaternary, binary |
| Flow Rate Range | 0.001 to 10 mL/min |
| Detection Methods | UV-Vis, Fluorescence, Electrochemical, MS |
| Temperature Control | Yes, with column oven |
| Sample Capacity | Varies by autosampler model |
| pH Range | 1 to 14 |
| Injection Volume | 0.1 to 100 µL |
| Detection Wavelength Range | 190 to 800 nm |
| Autosampler Capacity | Varies by model, up to several hundred samples |
| Dimensions (H x W x D) | Varies by module |
| Weight | Varies by configuration |
| Power Requirements | 100 - 240 VAC, 50/60 Hz |
Details symbols, precautions, and warnings for safe operation and handling of chemical solvents and the HPLC system.
Details key features: Rear Seal Wash, Flow Meter, Leak Sensor, Purge Valve, Inline Filter.
Explains how to control the module using the Chromeleon Chromatography Management System.
Covers features for monitoring module health, performance, and diagnostics.
Lists requirements for the installation site, including access, stability, and environmental conditions.
Covers loading USB drivers, installing, and configuring the module within Chromeleon.
Details rear seal wash setup, flow meter settings, and pump purging procedures.
Explains the process of equilibrating the UltiMate 3000 system before sample analysis.
Describes module power-up sequence, internal tests, and status indicators.
Guides on connecting, direct control, and automated control via Chromeleon.
Covers solvent selection, flow/pressure limits, purging, leak detection, and degasser operation.
Details temperature control, column ID, sensor sensitivity, and screen adjustments.
Covers predictive performance, diagnostics, digital I/O, and qualification functions.
Provides best practices for achieving optimal system stability and reproducibility.
Lists precautions for interrupting operation or preparing the module for shipping.
Details general maintenance, intervals, and calibration procedures for optimal performance.
Introduces features for identifying and eliminating operational problems.
Lists module-related messages and their corresponding remedial actions.
Lists common operating problems, their probable causes, and suggested remedial actions.
Covers general service notes, safety precautions, and procedures for user-performed maintenance.
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