Thermo Scientific Dionex UltiMate 3000 Series User Manual (English)

Questions and Answers:

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Gerald GreenAug 27, 2025
What to do if Thermo Scientific Laboratory Equipment can't reset pressure sensor because pressure is out of range?
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  rherreraAug 27, 2025
  If the pump pressure is not down to zero during the self test, wait until the pressure is down and repeat the test. Verify that the cable for the system pressure transducer is properly connected to the P-Sys connector for the loading pump on the interior front panel. Open and close the purge valve if necessary to reduce the pressure.
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Megan BeltranAug 27, 2025
What to do if Thermo Scientific Dionex UltiMate 3000 can't reset pressure sensor because pressure is not constant?
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  Robert MillerAug 27, 2025
  If the pump pressure is not down to zero during the self test, wait until the pressure is down and repeat the test. Verify that the cable for the system pressure transducer is properly connected to the P-Sys connector for the loading pump on the interior front panel. Open and close the purge valve if necessary to reduce the pressure.
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Rebecca SnyderAug 27, 2025
Why flow calibration failed with Implausible measurement results in Thermo Scientific Dionex UltiMate 3000 Laboratory Equipment?
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  Melissa JohnsonAug 27, 2025
  If flow calibration of the ProFlow flow meter yields implausible measurement results, a mismatch between the solvent and the sensor selected for calibration was detected. Repeat the flow calibration with the correct solvent or change the solvent. Contact Service.
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Yesenia RiveraAug 29, 2025
What to do if Thermo Scientific Dionex UltiMate 3000 displays 'Flow meter missing'?
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  tyangAug 29, 2025
  If communication with the flow meter failed, verify that the flow meter is installed properly.
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Angela BushAug 27, 2025
Why Thermo Scientific Dionex UltiMate 3000 cannot deliver desired flow at this pressure?
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  Juan BishopAug 27, 2025
  If the internal limit for the primary pressure has been reached, preventing the set flow rate from being delivered, shifts in retention times may be observed during an analysis. Verify that the column pressure is within the normal range. If it is, reduce the flow rate to avoid the problem.
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Justin RamirezAug 26, 2025
What to do if Thermo Scientific Dionex UltiMate 3000 can't start pump while alarm is on?
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  Tyler FowlerAug 26, 2025
  If an alarm has occurred, such as a leak alarm, you must acknowledge the alarm before restarting the pump flow. For example, perform the AlarmOff command in Chromeleon.
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joshua56Aug 26, 2025
Why has the pressure fallen below the lower limit in Thermo Scientific Dionex UltiMate 3000?
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  Dr. Jill Carlson DDSAug 26, 2025
  If the pressure has fallen below the lower limit, it may be because the solvent reservoirs are empty. Fill the reservoirs and purge the pump. Also, check the solvent supply line for air bubbles, inspect the filter frits, and purge the system.
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elizabethorozcoAug 26, 2025
What does it mean when the LED flashes red on Thermo Scientific Dionex UltiMate 3000 Laboratory Equipment?
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  Tracey SheppardAug 26, 2025
  If the LED flashes red, it indicates that the pistons are not in the correct position for normal operation, possibly after piston replacement, or an error occurred during internal maintenance. To resolve this: 1. Open the purge valve. 2. Perform the Dock Pistons command. 3. Close the purge valve.
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greynoldsAug 25, 2025
Why calibration procedure failed in Thermo Scientific Dionex UltiMate 3000?
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  rothbreannaAug 26, 2025
  If the calibration procedure fails because pressure cannot be built up and held, consider the following possible causes: * Ensure the purge valves are closed. * Verify that the fitting plug is correctly installed. * Check for leaks in the system. Find and eliminate any leaks, tightening any loose connections. Afterward, repeat the command.
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Dr. Casey Chavez PhDAug 25, 2025
What to do if Thermo Scientific Dionex UltiMate 3000 cannot pressurize the system?
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  Paula BakerAug 26, 2025
  If pressure buildup fails, the possible causes and solutions are: * Check for air bubbles in the system and purge the pump. * If the inlet valve is defective, replace the valve cartridge. * If the solvent reservoir is empty, fill it and then purge the pump.

Thermo Scientific Dionex UltiMate 3000 Series Specifications

General

Type	HPLC System
Data Sampling Rate	up to 100 Hz
Pump Type	Quaternary, binary
Flow Rate Range	0.001 to 10 mL/min
Detection Methods	UV-Vis, Fluorescence, Electrochemical, MS
Temperature Control	Yes, with column oven
Sample Capacity	Varies by autosampler model
pH Range	1 to 14
Injection Volume	0.1 to 100 µL
Detection Wavelength Range	190 to 800 nm
Autosampler Capacity	Varies by model, up to several hundred samples
Dimensions (H x W x D)	Varies by module
Weight	Varies by configuration
Power Requirements	100 - 240 VAC, 50/60 Hz