Why is the fluorescence extremely bright and bleeding through into other filters on my Thermo Scientific Invitrogen Countess 3 FL Laboratory Equipment?
If the fluorescence appears extremely bright and is bleeding through into other filters on your Thermo Scientific Laboratory Equipment, the light intensity might not be set correctly. You should decrease the fluorescence light intensity before counting the cells.
General| Brand | Thermo Scientific |
|---|---|
| Model | Invitrogen Countess 3 FL |
| Category | Laboratory Equipment |
| Language | English |
Details the features and capabilities of the Countess™ 3 FL Automated Cell Counter.
Covers initial inspection, part verification, and instrument registration procedures.
Identifies and describes the external components and ports of the instrument.
Specifies environmental requirements and steps for installing the instrument.
Guides through the first-time power-on and setup screens of the instrument.
Explains how to link the instrument to a new Thermo Fisher Connect account.
Describes the process for logging into an existing Thermo Fisher Connect account.
Introduces the interface for creating, loading, and managing custom templates.
Covers actions like edit, import, export, delete, and parameter adjustments.
Provides instructions on loading, creating, and editing custom templates for cell counting.
Details the process for importing templates from Cloud or USB.
Details the process for exporting templates to Cloud or USB.
Explains how to remove custom templates from the instrument.
Offers guidelines for optimal cell sample preparation and handling.
Step-by-step guide for preparing and loading disposable Countess™ slides.
Instructions for preparing and loading the Countess™ Reusable Slide for cell counting.
Covers the primary method for counting cells using brightfield illumination.
Outlines actions after a count, like identifying cells, viewing histograms, gating, and saving.
Explains how to interpret results displayed on the screen after a brightfield count.
Describes how to identify and review counted cells on the results screen.
Details how to visually distinguish and mark live vs. dead cells in the results.
Explains how to view cell distribution based on size for live/dead cells.
Covers adjusting count parameters (size, brightness, circularity) using gating controls.
Instructions for saving adjusted count parameters as new or updated templates.
Introduces the use of EVOS™ light cubes for various fluorescence applications.
Step-by-step guide for performing cell counts using fluorescence assays.
Details how to adjust exposure settings for fluorescence channels.
Explains how to select channels and initiate the count for fluorescence assays.
Actions to take after a fluorescence count, including analysis and saving.
Explains how to interpret results, identify cells, and perform further analysis.
How to identify and review cells counted in specific fluorescence channels.
Viewing cell distribution by size or fluorescence intensity for fluorescence assays.
Adjusting fluorescence gating parameters like intensity and size.
How to save custom parameter settings for fluorescence assays as templates.
Tool to calculate sample dilutions before loading for counting.
Tool to determine sample and buffer amounts for desired cell concentrations.
Details available options (Result, Images, CSV, Report, FCS) and steps for saving data.
How to create a printer-friendly PDF report of assay results, graphs, and images.
Navigating settings and connecting the instrument to a wireless network.
Steps for updating the instrument's software via USB or Connect account.
Instructions on how to configure the instrument's date and time settings.
How to select and change the instrument's cloud region.
Step-by-step guide for installing or replacing EVOS™ light cubes.
Guidelines for maintaining the instrument and using the correct power supply.
General guidance on cleaning the instrument to maintain performance.
Specific instructions for cleaning the instrument's touchscreen display.
Procedures for cleaning the exterior case of the instrument.
Methods for decontaminating the instrument, including using 70% alcohol.
Addresses issues with uneven screen illumination and autofocus problems.
Solutions for cells appearing in images but not included in counts.
Advice for issues with extremely bright fluorescence and bleeding.
Addresses incorrect concentration readings and variable counts due to pipetting.
Solutions for variability in replicate counts and issues with slide handling.
Guidance on accurate identification of live vs. dead cells and effects of trypan blue exposure.
Steps to resolve issues where the USB drive is not recognized by the instrument.
Solutions for problems encountered during software update processes.
Details the physical dimensions, weight, power, and environmental operating conditions.
Lists processing time, concentration ranges, sample volume, firmware, and USB drive compatibility.
Information on the instrument's optical channels and camera specifications.
Details the material, dimensions, and chamber volume of the analysis slides.
Explains the LED light source and introduces the concept of EVOS™ light cubes.
Lists the main instrument and its accessories with catalog numbers.
Lists consumable accessories like slides and stains with catalog numbers.
Explains the CSV format and defines general data categories and columns.
Details the CSV columns related to fluorescence assay data.
Defines CSV columns for brightfield analysis, such as cell size, brightness, and circularity.
Outlines essential safety practices for laboratory personnel using the instrument.
Provides guidelines for safe handling of chemicals, waste, and potential biohazards.
Addresses potential biohazards and necessary precautions when working with biological samples.
Information on how to contact support, access FAQs, and find product documentation.
Details regarding the warranty terms and conditions for the product.
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