10. Heating of the sample occurs with long runs. Gross sample heating can be
minimized by:
A. Nesting the sample vessel in an outer vessel containing ice.
B. Select longer interval (gap) times.
C. Using a specially fabricated leptosomatic ultra-sonication vessel
(available as an accessory).
The user should keep in mind that transient (millisecond) heating and free
radical formation in the immediate vicinity of the ultrasonic probe tip is
unavoidable. Please take this into consideration when working with samples
especially sensitive to heat or free radical damage. To avoid excessive
cavitation leading to overload when using smaller ultrasonic probes of 2,
3, and 6 mm tip diameter, the ultrasonic power wheel should be rotated to a
lower power setting.
11. If the liquid volume containing the sample is less than 5 ml, choose an
ultrasonic probe with a tip diameter of 2 to 3 mm. Smaller probes are
inserted into liquid at a depth about 1 cm,and the distance between the tip of
the ultrasonic probe and container bottom will vary to some extent by the
power setting selected for the probe. However, it should not be less than 0.5
cm.
X. Warnings and Notes continued
For questions or concerns, e-mail service@ussolid.com, visit www.ussolid.com,
or call (800) 243-5428
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