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Varian 380-LC - User Manual

Varian 380-LC
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Service Manual
FOR
Varian 380-LC & 385-LC
EVAPORATIVE
LIGHT-SCATTERING
DETECTOR
Version 1.0 January 2008
PL0890-0360
Polymer Laboratories Ltd, Now a part of Varian, Inc., Essex Road, Church Stretton,
Shropshire SY6 6AX, UK
Tel +44 (0)1694 723581, Fax +44 (0)1694 722171, Service Tel +44 (0)1694 724333
Polymer Laboratories, Varian, Inc., Amherst Fields Research Park, 160 Old Farm Road,
Amherst, MA 01002, USA Tel +1 413 253 9554, Fax +1 413 253 2476
Polymer Laboratories, Varian B.V., Herculesweg 8, 4339 PL Middelburg,
The Netherlands Tel +31 (0)118 671500, Fax +31 (0)118 671502
Polymer Laboratories, Varian Belgium NV SA, Mechelsesteenweg 362,
2860 St.-Katelijne-Waver, Belgium Tel +32 (0) 15 556460, Fax +32 (0) 15 556186
Polymer Laboratories, Varian Deutschland GmbH, Alsfelder Straße 6,
D-64289 Darmstadt, Germany Tel: +49 (0)6151 7030 Fax: +49 (0)6151 703237
Varian S.A.,7 Avenue des Tropiques, Z.A. Courtaboeuf, B.P. 12
F-91941 Les Ulis Cedex, France Tel +33 1.6986.3838 Fax: +33 1.6928.2308

Table of Contents

Questions and Answers

  • D
    Daniel SpearsAug 15, 2025
    What to do if Varian 380-LC Security Sensors detect a leak inside the unit?
    • A
      Anna LeeAug 15, 2025
      If the Varian Security Sensors detect a leak inside the unit, it means that solvent vapor has been detected. The suggested solution is to stop the pump.
  • E
    Elizabeth WhiteAug 18, 2025
    What should I do if the Varian 380-LC' liquid leak sensor has detected liquid in the drip tray?
    • C
      Christopher HernandezAug 18, 2025
      If the liquid leak sensor in your Varian Security Sensors has detected liquid in the drip tray, it indicates liquid is in the base of the unit. You should stop the pump and investigate the cause.
  • J
    Jennifer CrawfordAug 22, 2025
    Why is there baseline noise in Varian Security Sensors and how to fix it?
    • T
      tiffanyreyesAug 22, 2025
      Baseline noise in Varian Security Sensors can occur if the particle plume is not sufficiently dried in the evaporator tube. To resolve this, increase the temperature of the evaporator by 10° intervals until the noise is acceptable.
  • A
    adamsmatthewAug 26, 2025
    Why is there baseline noise in Varian Security Sensors and how can I resolve it?
    • K
      Kelsey MclaughlinAug 26, 2025
      Baseline noise in Varian Security Sensors can be caused by poor nebulisation of solvent. Increasing the gas flow rate should resolve this issue.
  • W
    weberchristopherAug 28, 2025
    Why is there baseline noise in Varian Security Sensors due to pump pulsations, and what is the solution?
    • S
      Sharon EllisAug 28, 2025
      Baseline noise in Varian Security Sensors can be caused by pump pulsations, especially in microbore applications where low flow rates are used. To fix this, increase the back pressure on the pump by fitting a back pressure regulator between the pump and the injection valve.
  • K
    Katherine RichardsonSep 1, 2025
    What causes a Fan Driver IC Thermal Shutdown in Varian 380-LC?
    • H
      Heather MartinSep 1, 2025
      A Fan Driver IC Thermal Shutdown in Varian Security Sensors can be caused by a fault with the circuitry, wires, or the fan itself. No specific solution is provided.
  • C
    Craig JimenezSep 4, 2025
    Why did the fan stop in Varian 380-LC?
    • S
      Sandra VazquezSep 4, 2025
      The fan in Varian Security Sensors may have stopped due to a fault with the circuitry, wires, or the fan itself. No specific solution is provided.
  • B
    Brent Joseph DVMSep 7, 2025
    Why is the air temperature less than or equal to 10°C inside the Varian Security Sensors enclosure?
    • K
      Kendra VargasSep 7, 2025
      If the air temperature inside the Varian Security Sensors enclosure is less than or equal to 10°C, it is because the environmental temperature is too cold. No specific solution is provided.
  • V
    Virginia SilvaSep 12, 2025
    Why is the air temperature greater than 40°C inside the Varian Security Sensors enclosure?
    • R
      Rebecca WebbSep 12, 2025
      If the air temperature inside the Varian Security Sensors enclosure is greater than 40°C, it is because the environmental temperature is too hot. No specific solution is provided.

Summary

Repairing the Varian ELSD

Overview of Main Assembly

Details the main components of the Varian ELSD detector for service access.

Removing The Front Panel

Step-by-step guide for removing the front panel for internal access and repairs.

Overview of Nebuliser Assembly

Describes the nebuliser assembly and its components, requiring front panel removal.

Removing The Nebuliser

Instructions for removing the nebuliser unit if it becomes blocked or requires replacement.

Installing The Nebuliser

Procedures for installing a new or cleaned nebuliser unit.

Replacing The Nebuliser Heater Assembly

Steps to replace the heater assembly within the nebuliser unit.

Replacing The Nebuliser Chamber

Guide for replacing the nebuliser chamber, often due to blockage or contamination.

Replacing The Solvent Leak Assembly

Steps to replace the sensor assembly that detects solvent leaks.

Replacing The Vapour Sensors

Instructions for replacing the external and internal vapour sensors.

Replacing The Fan Assembly

Guide for replacing the internal fan unit if it is faulty or noisy.

Overview of Varian 380-LC Evaporator Assembly

Details the evaporator assembly specific to the 380-LC model.

Removing And Cleaning The Evaporator Cartridge (Diffuser)

Steps for removing and cleaning the evaporator cartridge for sensitivity issues.

Replacing The Evaporator Heater Assembly (Evaporation Tube)

Guide for replacing the evaporator heater assembly or the evaporation tube.

Replacing The Mass Flow Controller

Instructions for replacing the mass flow controller (MFC) component.

Replacing The Gas Inlet Plug Assembly

Procedure for replacing the gas inlet plug assembly, usually for filter maintenance.

Replacing The Gas Inlet Assembly

Steps to replace the gas inlet valve assembly.

Replacing The Nebuliser Gas Regulator

Guide for replacing the gas regulator for the nebuliser.

Overview of Varian 385-LC Evaporator Assembly

Details the evaporator assembly specific to the 385-LC model.

Removing And Cleaning The Varian 385-LC Evaporator Cartridge (Diffuser)

Steps for removing and cleaning the evaporator cartridge for the 385-LC.

Replacing The Varian 385-LC Cooled Evaporator Assembly

Guide for replacing the cooled evaporator assembly on the 385-LC model.

Overview of Optical Assembly

Diagram and overview of the optical components of the ELSD.

Removing The Photodetector (PMT) Assembly

Steps for removing and replacing the Photodetector (PMT) module.

Adjusting Photodetector (PMT) Sensitivity

Procedure for adjusting the sensitivity of the PMT after replacement or change.

Replacing The LED Light Source Assembly

Guide for replacing the LED light source component.

Cleaning The Optical Lens Assembly

Instructions for cleaning the optical lenses within the assembly.

Replacing The Optical Light Trap

Steps for replacing the optical light trap component.

Replacing The Optical Heater

Guide for replacing the optical heater or its fuse.

Removing & Cleaning The Prism Assembly

Procedure for removing and cleaning the prism assembly.

Introduction

Safety

Installation

Site Preparation Check List

Lists environmental and power requirements before installing the detector.

Power Connections

Details how to connect the instrument to the mains power supply safely.

Gas Connection

Instructions for connecting the nitrogen gas supply to the instrument.

Fluid Connection

Steps for connecting the eluent from the chromatography system.

Extraction

Guidance on venting exhaust gases and vapors to a fume hood or disposal unit.

Data Connection

Information on connecting the detector for data acquisition via serial or analogue outputs.

Installing the Detector

Step-by-step guide for physically installing the detector and making connections.

Storing the Instrument

Procedure for properly storing the instrument when not in use.

Optimising Detector Performance

General Considerations

Discusses general setup precautions and solvent compatibility for optimal performance.

Optimisation Parameters

Details key parameters like gas flow, evaporator, and nebuliser temperatures.

Optimisation Procedure

Outlines the steps to adjust parameters for optimal detector performance.

Overview of Detectors Operation Modes

Standby Mode

Describes the "ground state" of the instrument for setup before operation.

Run Mode

Details the normal operational mode where the system is fully active.

Troubleshooting

Instrument Error Codes

A table listing error codes, their descriptions, and suggested solutions.

General Problems

Lists common operational problems, their causes, and remedies.

Electronics

Replacing The Main Control PCB

Steps for replacing the main control circuit board.

Replacing The Power Supply

Guide for replacing the instrument's power supply unit.

Firmware

Information on the detector's control firmware and its versions.

Device Identifier

Details on programmable device identification for controlling multiple detectors.

Upgrading Firmware

Step-by-step instructions for updating the instrument's firmware.

Identifying Parts and Materials

Instrument Specifications

Cleaning & Decontamination Procedures

Cleaning

Instructions for cleaning the exterior of the instrument.

Decontamination

Procedures for decontaminating the instrument after solvent spills.

Varian 380-LC Specifications

General IconGeneral
BrandVarian
Model380-LC
CategorySecurity Sensors
LanguageEnglish

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