• New columns contain a mixture of organic solvents and
water. See your QC Performance Report for the solvent
composition in your column. Initially, care should be taken
not to pass any mobile phase through the column that
may cause a precipitate to form.
• Agilent reversed-phase columns are compatible with water
and all common organic solvents.
• The use of a guard column is recommended to protect
your column and increase its lifetime.
• Columns should not be maintained at elevated pH or
elevated temperature when not in use.
• Avoid use of this column outside of recommended
pHranges for column phase (see next page).
Expect reduced lifetime when operating outside the
recommended pH and temperature ranges.
If you are using Poroshell 120 or ZORBAX RRHT or RRHD columns,
consider using a Fast Guard for UHPLC to protect your analytical
column. See agilent.com/chem/fastguards for more information.
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