Column operating parameters: pH and temperature
Note: All silica-based packings have some solubility in pH >6 aqueous
mobile phases. When using silica-based columns at pH >6, best column
lifetime is obtained at lower temperatures (40 °C max) using low buffer
concentrations in the range of 0.01 to 0.02 M. Operating at extreme ends
of pH and temperature ranges will have a signicant impact on column
lifetime.
Phase
Recommended
pH Range
Maximum Operating
Temperature
Poroshell HPH pH 3.0 to 11.0 60 °C
Poroshell 120 SB-C18,
StableBond C18,
ZORBAXRx-C8,
ZORBAX SB-Aq,
ZORBAX SB-Phenyl,
SB-C3, Poroshell 300,
300SB-C3
pH 1.0 to 8.0 90 °C (StableBond C18
and Poroshell 120 SB-C18)
80 °C (Rx-C8 and SB-Aq,
SB-C3 and SB-Phenyl)
70 °C for pH <5.0; 40°C
for pH 5.0 to 8.0 (Poroshell
300 and 300SB-C3)
ZORBAX Eclipse Plus C18
and C8, Eclipse XDB-C8,
XDB-C18, XDB-Phenyl,
Poroshell 120 EC-C18 and
EC-C8, ZORBAX Bonus-RP,
HC-C18(2) and TC-C18(2)
pH 2.0 to 9.0 60 °C
ZORBAX Extend C18 pH 2.0 to 11.5 60 °C (40 °C at high pH)
Poroshell 120 PFP,
Poroshell 120 Phenyl-Hexyl,
ZORBAX ODS,
ZORBAXPhenyl,
ZORBAXEclipse Plus
Phenyl-Hexyl,
ZORBAX XDB-CN,
ZORBAXEclipse PAH,
AllPursuit and Polaris
bonded phases
pH 2.0 to 8.0 60 °C
ZORBAX Eclipse PAH pH 2.0 to 8.0 60 °C at pH <6.0
ZORBAX TMS pH 2.0 to 7.0 60 °C