Agilent Technologies 6460 User Manual

Agilent 6400 Series Triple Quadrupole
LC/MS System
Quick Start Guide
Where to find information 3
Getting Started 5
Step 1. Start the Data Acquisition software 6
Step 2. Prepare the LC modules 12
Step 3. Prepare the Triple Quad instrument 16
Step 4. Set up and run an acquisition method 20
Step 5. Review results with the Qualitative Analysis program 27
Step 6. Analyze data with the Quantitative Analysis program 27
Use this guide for your first steps with the Agilent 6400 Series Triple Quad LC/MS, and as a
road map for your user information.
What is the Agilent 6400 Series Triple Quad LC/MS system?
The Agilent Triple Quadrupole LC/MS is a liquid chromatograph triple quadrupole
mass spectrometer that performs MS/MS using three sets of parallel rods (in this
case, quadrupole, hexapole, quadrupole). The first quadrupole separates ions into
precursor ions that are fragmented in the hexapole into product ions, which are
separated by the second quadrupole. Often, two or more precursor ions and their
product ions are monitored in sequence in MRM (multiple reaction monitoring)
mode. You can monitor up to 4000 MRM transitions by using Dynamic MRM.
The Agilent 6490 and 6495 Triple Quadrupoles contain the iFunnel Technology
which provide more efficient sampling of ions into the mass spectrometer. The
iFunnel Technology includes the Dual Offset Ion Funnel and these other
technologies: the Agilent Jet Stream Technology, and shorter desolvation assembly
with Hexabore Capillary.

Agilent Technologies 6460 Specifications

General

Brand	Agilent Technologies
Model	6460
Category	Laboratory Equipment
Language	English

Summary

Where to find information

Installation Guide

Guide to install hardware, configure instrument, and verify performance.

Upgrade Guide

Guide to upgrade MassHunter Workstation software.

Maintenance Guide

Guide to help maintain and troubleshoot the Agilent Triple Quad LC/MS.

Concepts Guide - The Big Picture

Background information for understanding hardware and software operation.

Data Acquisition for 6400 Series Triple Quad Familiarization Guide

Exercises to learn using LC/MS hardware and Data Acquisition program.

Study Manager Quick Start Guide

Guide to learn MassHunter Study Manager software.

MassHunter Optimizer Software Quick Start Guide

Guide to learn MassHunter Optimizer for automatic MRM parameter optimization.

Training

MassHunter QQQ Compliance Quick Start Guide

Guide to learn MassHunter QQQ Compliance program.

MassHunter Quant Compliance Quick Start Guide

Guide to learn MassHunter Quant Compliance program.

Qualitative Analysis Familiarization Guide

Exercises to learn Qualitative Analysis program.

Quantitative Analysis Familiarization Guide

Exercises to learn Quantitative Analysis program.

Familiarization Guides

Use all three familiarization guides to get to know the software.

Quick Start Guides

Use quick start guides for Study Manager, Optimizer and Compliance.

Training Courses

Visit website for training courses for Agilent Triple Quad LC/MS.

Getting Started

Install the Triple Quadrupole LC/MS hardware and software

Use the Installation Guide to install the hardware and software and verify performance.

Set up, run, and analyze samples

The roadmap shows steps to set up and run a batch of samples from start to finish.

Start the Data Acquisition software

Prepare the LC modules

Set up to condition or equilibrate the column

Type LC parameters and click Apply to download them to the LC.

Monitor the baseline and adjust the plot

Monitor baseline and adjust plot for column equilibration and stable baseline.

Prepare the Triple Quad instrument

Manual Tune

Click the Manual Tune tab to make quick changes to get Checktune to pass.

Autotune

Click Autotune to tune the MS automatically, adjusting parameters.

Set up and run an acquisition method

Enter the Triple Quadrupole parameter values

Select Scan Type and enter Acquisition values.

Set up to change Triple Quad MS parameters with segments and scans

Add a segment by right-clicking in Scan segments and typing parameters.

Review results with the Qualitative Analysis program

Analyze data with the Quantitative Analysis program