Agilent MassHunter Workstation –
Data Acquisition for 6400 Series Triple
Quadrupole LC/MS
Familiarization Guide
For In Vitro Diagnostic Use
Before you begin 3
Prepare your system 3
Prepare to acquire data 4
Exercise 1 – Develop an acquisition method 6
Task 1. Enter acquisition parameters and acquire data 6
Task 2. Determine precursor ion masses 11
Task 3. Find optimum fragmentor voltage for maximum response 14
Task 4. Determine product ion masses 24
Task 5. Find optimum collision energy for MRM acquisition 30
Exercise 2 – Develop a Dynamic MRM method from an MRM acquisition
data file or an MRM method 33
Task 1. Create a batch file from an existing MRM data file 33
Task 2. Print a report in the Quantitative Analysis program 36
Task 3. Create a Dynamic MRM method using Update dMRM 38
Task 4. Create a Dynamic MRM method from an MRM method 40
Exercise 3 – Create a Triggered Dynamic MRM acquisition method 42
Task 1. Create a Triggered Dynamic MRM method from a Dynamic
MRM method manually 42
Task 2. Add/Modify compounds in an existing database 45
Task 3. Create a Triggered Dynamic MRM method from an existing
database 55
Exercise 4 – Optimize Acquisition parameters 59
Task 1. Use Optimizer to optimize acquisition parameters 59
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