Agilent Technologies 6495 - User Manual

Agilent 6400 Series Triple Quadrupole
LC/MS System
Quick Start Guide
Where to find information 3
Getting Started 5
Step 1. Start the Data Acquisition software 6
Step 2. Prepare the LC modules 12
Step 3. Prepare the Triple Quad instrument 16
Step 4. Set up and run an acquisition method 20
Step 5. Review results with the Qualitative Analysis program 27
Step 6. Analyze data with the Quantitative Analysis program 27
Use this guide for your first steps with the Agilent 6400 Series Triple Quad LC/MS, and as a
road map for your user information.
What is the Agilent 6400 Series Triple Quad LC/MS system?
The Agilent Triple Quadrupole LC/MS is a liquid chromatograph triple quadrupole
mass spectrometer that performs MS/MS using three sets of parallel rods (in this
case, quadrupole, hexapole, quadrupole). The first quadrupole separates ions into
precursor ions that are fragmented in the hexapole into product ions, which are
separated by the second quadrupole. Often, two or more precursor ions and their
product ions are monitored in sequence in MRM (multiple reaction monitoring)
mode. You can monitor up to 4000 MRM transitions by using Dynamic MRM.
The Agilent 6490 and 6495 Triple Quadrupoles contain the iFunnel Technology
which provide more efficient sampling of ions into the mass spectrometer. The
iFunnel Technology includes the Dual Offset Ion Funnel and these other
technologies: the Agilent Jet Stream Technology, and shorter desolvation assembly
with Hexabore Capillary.

Agilent Technologies 6495 Specifications

General

Type	Triple Quadrupole LC/MS
Mass Analyzer	Triple Quadrupole
Ionization Source	Electrospray Ionization (ESI)
Polarity Switching	20 ms
Resolution	Unit resolution (0.7 FWHM)
Ion Source	Agilent Jet Stream Technology (AJS) ESI and APCI

Summary

Getting Started

Set up, run, and analyze samples

Roadmap for setting up and running samples from start to finish.

Step 1. Start the Data Acquisition software

Windows-where you do most of your work

Overview of the main windows used for acquisition tasks.

Instrument Status Window

View the status of each device configured with the instrument.

Method Editor Window

Enter acquisition parameters for the method.

Sample Run Window

Enter sample information to run individual samples.

Step 2. Prepare the LC modules

Step 3. Prepare the Triple Quad instrument

Do a Checktune, Autotune or Manual Tune

Guide to performing instrument tuning procedures.

Step 4. Set up and run an acquisition method

Set up the method

Details on configuring acquisition method parameters.

Set up data analysis (DA) parameters

Configure parameters for data analysis within the method.

Set up and run worklists

Steps for configuring and executing sample worklists.

Step 5. Review results with the Qualitative Analysis program

Step 6. Analyze data with the Quantitative Analysis program