Agilent Technologies 6490 User Manual

Agilent 6400 Series Triple Quadrupole
LC/MS System
Quick Start Guide
Where to find information 3
Getting Started 5
Step 1. Start the Data Acquisition software 6
Step 2. Prepare the LC modules 12
Step 3. Prepare the Triple Quad instrument 16
Step 4. Set up and run an acquisition method 20
Step 5. Review results with the Qualitative Analysis program 27
Step 6. Analyze data with the Quantitative Analysis program 27
Use this guide for your first steps with the Agilent 6400 Series Triple Quad LC/MS, and as a
road map for your user information.
What is the Agilent 6400 Series Triple Quad LC/MS system?
The Agilent Triple Quadrupole LC/MS is a liquid chromatograph triple quadrupole
mass spectrometer that performs MS/MS using three sets of parallel rods (in this
case, quadrupole, hexapole, quadrupole). The first quadrupole separates ions into
precursor ions that are fragmented in the hexapole into product ions, which are
separated by the second quadrupole. Often, two or more precursor ions and their
product ions are monitored in sequence in MRM (multiple reaction monitoring)
mode. You can monitor up to 4000 MRM transitions by using Dynamic MRM.
The Agilent 6490 and 6495 Triple Quadrupoles contain the iFunnel Technology
which provide more efficient sampling of ions into the mass spectrometer. The
iFunnel Technology includes the Dual Offset Ion Funnel and these other
technologies: the Agilent Jet Stream Technology, and shorter desolvation assembly
with Hexabore Capillary.

Agilent Technologies 6490 Specifications

General

Type	Triple Quadrupole LC/MS
Dynamic Range	> 5 orders of magnitude
Polarity Switching	Yes
Polarity Switching Speed	20 ms
Mass Range	m/z 5 to 3, 000
Ion Source	Agilent Jet Stream
Data Acquisition Rate	Up to 500 per second
Resolution	0.7 Da at unit resolution
Mass Accuracy	0.1 amu

Summary

What is the Agilent 6400 Series Triple Quad LC/MS system?

What's New in Data Acquisition

Where to find information

Help

Access help via F1 key or Help menu for software details and guidance.

Documents

Lists available manuals for installation, upgrade, maintenance, and guides.

Training

Information on training courses and guides for software programs.

Getting Started

Start the Data Acquisition software

Step 1: Initiate the Data Acquisition program and explore its interface.

Prepare the LC modules

Step 2: Prepare LC modules, including switching to waste and purging.

Prepare the Triple Quad instrument

Step 3: Prepare the Triple Quad instrument by performing Checktune and Autotune.

Set up and run an acquisition method

Step 4: Set up and run acquisition methods, including parameters and worklists.

Review results with the Qualitative Analysis program

Step 5: Review acquisition results using the Qualitative Analysis program.

Analyze data with the Quantitative Analysis program

Step 6: Analyze acquired data using the Quantitative Analysis program.