Exercise 1 – Develop an acquisition method
Task 1. Enter acquisition parameters and acquire data
6 Agilent 6400 Series Triple Quad LC/MS Familiarization Guide
Exercise 1 – Develop an acquisition method
For this exercise you analyze a mixture of four sulfonamide compounds. These
tasks show you how to manually select the acquisition parameters including
using the Qualitative Analysis program to analyze the data files. You can
instead use the automated process to select some of the acquisition
parameters. See “Exercise 4 – Optimize Acquisition parameters” on page 59 to
learn how to automate this process.
Task 1. Enter acquisition parameters and acquire data
In this exercise, you enter the conditions for the analysis of the sulfa drug mix.
l
Steps Detailed Instructions Comments
1 Enter LC parameters appropriate
for sulfa drug mix.
See Table 2.
a Double-click the Data Acquisition
icon.
b Make sure that Acquisition appears
as the selection in the Context text
box.
If Tune is the selection, click
Acquisition from the Context
dropdown menu in the Combo bar.
c Enter the LC parameters listed in the
Table 2.
• The Data Acquisition window
appears. See Figure 1 on page 8.
Table 2 LC parameters for sulfa drug mix
Parameter LC Parameter
PUMP
• Flowrate 800 µL/min
• Solvent A 5 mM ammonium formate in water
• Solvent B 5 mM ammonium formate in 90:10 acetonitrile:water
• Gradient (min - %B) 0 min - 13%
1.80 min - 60%
2 min - 60%
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