Rev 05/12/21 pg. 15
ADF Analysis Procedure using the ANKOM
2000
Fiber Analyzer
If you are following the acid detergent procedure with an acid detergent lignin,
you can find the lignin procedure on our website at www.ankom.com.
To perform ADF analysis on prepared samples, follow the procedure detailed below.
If you are changing from one analysis type to another, flush the system before
running. See the “Flush Procedure” section of this manual for more
information.
Verify that the hot water supply is on and the drain hose is securely positioned in the drain.
If you are using Cubitainers for your chemicals, attach the AD solution
hose first to the Cubitainer and then to Port B on the instrument.
The solutions are gravity fed into the instrument ports. The bottom of the
solution level must be at least 5” (13cm) above the port. The top of the
solution level can be no more than 20” (50cm) above either port or the
solution will overflow into the drain.
Place the Bag Suspender with the samples into the Vessel.
Place the Bag Suspender Weight onto the Bag Suspender rod to hold the trays in place.
Turn the instrument Power Switch to the ON position. The Display will light and allow you to select an
analysis procedure.
Press the arrow keys on the Keypad until you see “Select ADF” on the
Display.
The ADF analysis will use the following default settings:
• 60 minute digestion
• four 5 minute rinses
If you want to run a custom ADF analysis that allows you to set the digestion
time and the number of rinses, press the arrow keys until you see “Select
Custom” on the Display.
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