BD FACSFlow Supply System - Page 32

BD FACSFlow Supply System User’s Guide32
Flushing the system To flush the system:
1. Turn on the BD FACSFlow supply system.
2. Fill the stainless steel container with 2 L of 10% bleach
solution (or full-strength BD FACSClean solution).
3. Open the roller clamp on the fluidics line for about 5 seconds
and drain the fluid into a beaker. This runs bleach up into the
fluid line.
4. Remove the DI water tube from the SIP and leave the support
arm to the side.
5. Prime the cytometer twice:
a. Press the PRIME button on the fluidics control panel.
b. When the STNDBY button lights (amber), press the
PRIME button again.
6. Install a tube with 3 mL of 10% bleach or full-strength BD
BD FACSClean solution on the SIP and put the tube support
arm underneath the tube.
7. Press RUN and HI on the cytometer fluid control panel. Run
for 30 minutes.
8. Press the STNDBY fluid control button, disconnect the air line
at the stainless steel container, and depressurize the stainless
steel container by lifting the vent valve.
9. Dispose of any remaining solution and rinse the container with
DI water.
10. Empty the waste tank.
11. Reconnect the air line to the stainless steel container.
12. Repeat steps 2 through 11 with DI water.
Caution! Confirm that the sheath filter is not in the
fluid line. Bleach will damage the filter.