Section 11
Specifications
11.1 Specifications
Product Specifications SmartSpec 3000 Spectrophotometer
Wavelength range: UV and Visible ranges 200 to 800 nm
Wavelength accuracy (+/-) 2 nm
Spectral bandwidth 5nm
Light source Xenon flash lamp
Detector Photodiode array
Number of cuvettes one
Size of cuvettes 100 µl, 700 µl, 1.5 ml, 3.5 ml
Pathlength 1 mm, 2 mm, 5 mm and 10 mm
Display 2 x 24 alphanumeric built-in backlit LCD
display
Printer Built-in alphanumeric/graphic
Interface RS-232 serial port
Operating temperature 5–40 °C
Photometric range 0–2 AU
Scan speed 200–800 nm in 20 seconds max
Photometric repeatability ± 0.02 AU in a 0.1–2.0 AU range
(9 readings) and over a 200–800 nm range.
Dimensions 33cm(W)x26cm(D)x14cm(H)
Weight 3.6 k.g.
Power requirement 90–260 VAC, 47–63 Hz
Regulatory CE, TÜV
11.2 Calculations
I. Molecular Weight (MW):
• Length (L): MW ≈ L*310-61
• Sequence or Composition:
DNA: MW = (#A * 312.2) + (#C * 288.2) + (#G * 328.2) + (#T * 303.2) - 61
RNA: MW = (#A * 312.2) + (#C * 288.2) + (#G * 328.2) + (#U * 289.2) - 61
II. Molar Extinction Coefficient (
εε
):
• Length: ε ≈ L * 10,000 liter/mole/cm
• Composition:
DNA: ε ≈ (#A * 15480) + (#C * 7340) + (#G * 11760) + (#T * 8850) liter/mole/cm
RNA: ε ≈ (#A * 15480) + (#C * 7340) + (#G * 11760) + (#U * 9000) liter/mole/cm
• Sequence: See Optical properties of nucleic acids, absorption, and circular dichroism
spectra, p. 589, CRC Handbook of Biochemistry and Molecular Biology 3rd Edition,
Nucleic Acids - Volume I. Gerald D. Fasman, Ed. CRC Press 1975.
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