BIO RAD Trans-Blot Turbo

Questions and Answers

D
David NguyenJul 27, 2025
What to do if NO LOAD DETECTED is displayed on the Run screen of my BIO RAD Trans-Blot Turbo Laboratory Equipment?
- C
  Christopher Smith MDJul 27, 2025
  If 'NO LOAD DETECTED' appears, it indicates that the instrument detects a cassette, but current isn't passing between the electrode plates. Ensure the blotting assembly is correctly positioned and thick enough to contact both electrode plates. If using a custom buffer, verify the concentrations of its components. Also, with the instrument unplugged, clean the electrical contacts with a cotton swab or wipe moistened with isopropyl alcohol.
L
Luke SuttonJul 31, 2025
What to do if POWER FAILURE! PRESS ANY KEY TO CONTINUE is displayed on the screen of my BIO RAD Trans-Blot Turbo?
- N
  newmanthomasJul 31, 2025
  If the message 'POWER FAILURE! PRESS ANY KEY TO CONTINUE' appears, it means the instrument lost power during operation. Turn off the power switch, restore power, and then restart the instrument.
R
Robin FrancoAug 4, 2025
Why is there poor or no electrophoretic transfer with BIO RAD Trans-Blot Turbo Laboratory Equipment, and excessive protein remains in the gel?
- N
  Nicholas MillerAug 5, 2025
  Poor electrophoretic transfer with excessive protein remaining in the gel can occur due to several reasons: * The transfer time may be too short. Try increasing the transfer time, or adjusting the percentages of the components for handcast gels. * The wrong protocol was used for the gel combination. Refer to Table 1 for acceptable gel combinations. * Too much protein was loaded on the gel. Reduce the amount of protein used. * Optimization might be needed for some proteins. Increase the transfer time in 1 minute increments, and adjust voltage and current if necessary.
C
carternatalieAug 9, 2025
Why is there poor or no electrophoretic transfer with BIO RAD Laboratory Equipment, and little or no protein remains in the gel?
- J
  Jenny WilliamsAug 9, 2025
  If you experience poor or no electrophoretic transfer, and little to no protein remains in the gel, it could be due to the blotting sandwich being assembled incorrectly. Refer to Figure 8 for the correct assembly. Alternatively, the detection system may not be working or is not sensitive enough. Include proper antigen controls to test detection kit sensitivity or use a total protein stain or Criterion Stain Free™ system to detect protein transfer to the membrane prior to immunodetection.
J
Julie CarterAug 13, 2025
How to fix swirls or missing bands with BIO RAD Laboratory Equipment?
- R
  Rebecca WilliamsAug 13, 2025
  Swirls, missing bands, or diffuse transfers can arise from several issues. Ensure good contact between the membrane and gel by using a blot roller to remove air bubbles or excess buffer. If using conventional semi-dry consumables, improve contact between the assembled sandwich and electrodes with extra-thick filter paper or additional buffer-wetted filter paper. Avoid letting the membrane dry out, as white spots indicate areas where protein won't bind. Adjust voltage and current if power conditions are too high for your gel. If using a single mini transfer pack, make sure it's near the center of the cassette base. When transferring two mini gels simultaneously, arrange them foot-to-foot.
K
kmannAug 17, 2025
What to do if the blot sandwich exceeds 70ºC after transfer using BIO RAD Trans-Blot Turbo?
- J
  Jonathan RamosAug 17, 2025
  If the blot sandwich exceeds 70ºC after transfer, it may be due to using an incorrect program for the gel combination. Check Table 1 for acceptable gel combinations. You can also try reducing the run current, voltage, or time.
E
Edward GordonAug 22, 2025
What to do if the cassette does not stay in place in the instrument bay of my BIO RAD Laboratory Equipment?
- M
  Michael ChambersAug 22, 2025
  If the cassette doesn't stay in place, ensure the dial on the cassette lid is in the Lock position and the lid pins are engaged. The dial should face up when inserting the cassette. If the magnetic interlock is damaged, contact Technical Support for assistance.