BIO RAD NGC User Manual

2
Connect the power cable and USB to the back of the FC.
Plug in the power supply and connect the USB cable into
a USB port on the NGC Chromatography System.
Introduction
The NGC Fraction Collector provides versatile options for
preparative and analytical chromatography applications. The
high-capacity and flexible rack options enable fractions ranging
in size from milliliters to liters to be collected and accessed
freely during a purification run.
Setting Up the NGC Fraction Collector
This quick guide outlines the process of installing and plumbing
the NGC Fraction Collector to your NGC System. The fraction
collector (FC) can be installed on a laboratory bench or in a
coldroom or chromatography refrigerator.
1
Open the shipping box and remove the foam packaging.
Carefully lift the FC from the box. Do not lift the FC by the
gantry; reach down and lift it from the base. Grip the base
of the FC by the sides to lift from the shipping box.
Check the box for the tubing kit, external power supply,
and USB cable. The tubing kit contains:

92 cm green polyetheretherketone (PEEK) tubing
from NGC union to dispense head for F100 systems
(fittings on both ends)

92 cm orange PEEK tubing for F10 systems (fittings
on both ends)

92 cm clear fluorinated ethylene propylene (FEP)
waste line from dispense head to union (fittings on
both ends)

150 cm clear FEP waste line from union to waste
bottle (fitting on one end)

Black magnetic union to be attached to side of NGC
System to act as anchor point for FC tubing; position
as necessary to avoid kinking any of the tubes
Installing the NGC Fraction Collector
CHROMATOGRAPHY SYSTEMS
NGC
™
Fraction Collector Installation
Quick Guide
Dispense head height adjustment
Standard racks
USB port Power port
Drip tray
Dispense head cover
Gantry

BIO RAD NGC Specifications

General

Type	Chromatography System
Application	Protein purification
Flow Rate Range	0.001 to 100 mL/min
UV Wavelength Range	190 to 800 nm
pH Range	2 to 12
Sample Injection	Automated sample injection
Temperature Control Range	4°C to 40°C
Software Compatibility	UNICORN

Summary

Installing the NGC Fraction Collector

Setting Up the NGC Fraction Collector

Outlines the process of installing and plumbing the NGC Fraction Collector to your NGC System.

Unboxing and Checking Components

Unpack the fraction collector, lift from the base, and check for tubing kit and power supply.

Connecting Power and Data Cables

Connect the power supply and USB cable from the FC to the NGC Chromatography System.

Plumbing the NGC Fraction Collector

Opening the Dispense Head Cover

Open the dispense head cover using a small Phillips screwdriver for access.

Connecting Sample and Waste Tubing

Connect sample tube to dispense head and waste line to union on NGC System.

Routing and Securing Tubing

Route tubes through the cover grommet, close cover, and fasten with screw.

Completing NGC Fraction Collector Installation

Placing Drip Tray and Rack Tray

Place the plastic drip tray and metal rack tray onto the FC platform correctly.

Powering On and Adjusting Z-Height

Turn on the fraction collector and adjust dispense head z-height for collection vessels.

Ordering Information

Accessory Catalog Numbers

List of catalog numbers and descriptions for related Peltier rack modules and tube racks.