BIO RAD S3e User Manual

Flow Cytometry/FACS – Bio-Rad S3e Cell Sorter

BIO RAD S3e Specifications

General

Type	Cell Sorter
Manufacturer	Bio-Rad
Model	S3e
Sample Throughput	Up to 10, 000 events per second
Data Acquisition Rate	Up to 30, 000 events per second
Nozzle Size Range	70 µm, 100 µm, 130 µm
Software	ProSort Software
Power Requirements	100–240 VAC, 50/60 Hz
Laser Options	488 nm, 561 nm
Laser Configuration	Up to 3 lasers
Detection Channels	Up to 6 fluorescence channels
Sample Volume	1.5 mL tubes, 5 mL tubes
Sample Input	1.5 mL tubes, 5 mL tubes
Sample Output	Tubes or plates
Fluidics System	Closed fluidic system with sheath fluid
Weight	Approximately 200 lbs (90.7 kg)
Detectors	Photomultiplier Tubes (PMTs)
Forward Scatter Detector	Silicon photodiode

Summary

Brief Description

Reference

Location

Suggestion for Materials and Methods

Suggestion for Acknowledgements

People and Contacts

Features and Specifications

Lasers and Filter-Sets

Additional Equipment

System Overview

Warnings and Advice

Available Techniques

Set Up

Sample Preparation

Prepares cells for sorting, ensuring single cell suspension and filtering.

Initial Set Up: Start-up and Fluidics Procedure

Initial Set Up: Quality Control

Final Set Up: Low-Pressure Cleaning

Final Set Up: Shut Down

Acquisition

Sorting

Other Guidelines

Swapping Fluidics During Sorting: Hot Swap

Auto Compensation Wizard

Protocols and Workspace

Create, Open, and Save Protocols

Manage protocols by creating new ones, opening existing, and saving changes.

Load Instrument Settings

Load pre-existing instrument settings from a protocol file.

Opening FCS Files

Further Protocols, Tutorials and Guidelines