8
WARNINGS and Advice
• Before you get started for the first time on this cell sorter consult the responsible
people for proper training
• For the moment, bacterial pathogens, cell lines or any other agent of Biosafety
level 2 or higher are not allowed, as well as radioactively labelled or flammable
samples
• Do not forget to check the fluidics before you start operating the cell sorter
• Use only MiliQ water in the water container
• While transferring a cap assembly to a new container, avoid touching the
assembly to the outside surfaces of the containers. If it is necessary to lay the cap
assemblies down, set only on pre-sterilized surfaces
• It is recommended that resuspended adherent samples are mixed at high speed
to prevent clumping
• Samples must be filtered prior to running. This will minimize clogs in the sample
tube and nozzle tip
• Do not open the nozzle door
• Keep the green containment door closed as often as possible during the sorting
procedure
• Never try to access the laser beam paths
• Any adjustment to the toolbars should be avoid. Only knowledgeable users must
perform alterations to the current settings. Please, refer to the responsible people for
any constraint
• Optimal settings are automatically determined during the quality control
procedure. Adjusting any of these values will affect the sorting performance
• Do not adjust any setting of the global preferences menu
• Please confirm the bead lot before starting the quality control procedure. If the
bead lot is not in the dropdown list, please refer to the responsible people
• Use always beads from the same lot to perform one quality control procedure
• When the remaining run time is down to 5 minutes, the system will automatically
shut down. The fluidics will need to be refilled and the start-up procedure must be
performed. To prevent this, perform the fluidics “hot swap”.
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