BIO RAD Mini-PROTEAN - User Manual

Remove tape
Remove comb
Reference line
to monitor run
Remove ta pe at botto m Remove ta pe at botto m
Align arrow on the
cassette with arrow
on the opening lever
Break at A rrowsBreak at A rrows
Opening the Cassette
Preparing the Cassette
Running the Gel
Instructions for Using Mini-PROTEAN Precast Gels
1. Remove Comb: Position thumb on indentation
(middle of comb) and remove comb by pulling
upward in one smooth motion.
2. Remove Tape: Pull the green tape gently to
remove it from the bottom of the cassette.
3. Assemble Mini-PROTEAN Tetra Cell:
Assemble the cassette into the running module
of the Mini-PROTEAN Tetra system. Add
running buffer to the inner and outer chambers.
Use a syringe or a disposable transfer pipet to
rinse the wells with running buffer.
4. Run Gel: Prepare the samples and load
into the wells. If using Bio-Rad Precision
Plus Protein
™
standards, load 10 µl (5 µl
for Precision Plus Protein
™
WesternC
™
standards). Run the gel until the dye front
reaches the reference line. Refer to the
instruction manual (#1658100) for more
information on running conditions. At the
completion of the run, disconnect the cell
and remove the cassette.
5. Open Cassette: Align the arrow on the
opening lever (catalog #456-0000) with the
arrows marked on the cassette. Insert the
lever between the cassette plates at the
indicated locations and apply downward
pressure to break the seal. Gently pull apart
the two plates beginning from the top of
the cassette.
Note: Opening lever is 100%
aluminum and recyclable.
6. Remove Gel: Gently remove the gel from
the cassette.
Please note: when running 1– 2 gels in the
Mini-PROTEAN Tetra cell, do not leave the
companion module in the tank; do not run
different gel types (chemistries) or percentages
at the same time.
ELECTROPHORESIS
Mini-PROTEAN
®
Precast Gels
Quick Start Guide

Summary

Instructions for Using Mini-PROTEAN Precast Gels

Remove Comb

Steps to remove the comb from the gel cassette by pulling upward.

Remove Tape

Steps to gently remove the green tape from the bottom of the gel cassette.

Assemble Mini-PROTEAN Tetra Cell

Assemble cassette, add running buffer to chambers, and rinse wells.

Run Gel

Prepare and load samples, run gel until dye front reaches reference line.

Open Cassette

Align lever with cassette arrows, apply pressure to break seal, and separate plates.

Remove Gel

Gently remove the gel from the opened cassette after the run is complete.

Ordering Information

BIO RAD Mini-PROTEAN Specifications

General

Brand	BIO RAD
Model	Mini-PROTEAN
Category	Laboratory Equipment
Language	English