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BIO RAD PTC-200 User Manual

BIO RAD PTC-200
113 pages
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DNA Engine
®
Thermal Cycler
Operations Manual
Version 4.0
PTC-200 DNA Engine Cycler

Table of Contents

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BIO RAD PTC-200 Specifications

General IconGeneral
BrandBIO RAD
ModelPTC-200
CategoryLaboratory Equipment
LanguageEnglish

Summary

Safety and Warnings

Explanation of Symbols

Identifies hazard symbols used in the manual and on the instrument.

Safety Warnings

Outlines critical hazards and precautions for operating the DNA Engine cycler.

Guidelines and Precautions

Chapter 1: Introduction

Meet the DNA Engine Thermal Cycler

Introduces the DNA Engine cycler and its key features.

Important Safety Information

Highlights essential safety precautions before operating the instrument.

Chapter 2: Layout and Specifications

Control Panel

Details the functions of the instrument's control interface.

Specifications

Lists the technical specifications for the DNA Engine thermal cycler.

Chapter 3: Installation

Chapter 4: Operation

Selecting the Correct Sample Vessel

Guidance on choosing appropriate vessels for the cycler's blocks.

Adjusting the Hot Bonnet Lid’s Pressure

Instructions for setting the correct pressure for the heated lid.

Chapter 5: Running Protocols

Choosing a Stored Protocol to Run

How to select a protocol from memory for execution.

Setting Up the Temperature Control Method

Configuring how the cycler controls temperature during a run.

Chapter 6: Creating Programs

Designing a New Program

Guides on creating new programs from scratch.

Choosing a Temperature Control Method

Explains the two methods: calculated and block control.

Entering a Gradient Step

How to program a temperature gradient across the block.

Chapter 7: Editing Programs

Editing the Program

Detailed steps and options for modifying program steps.

Chapter 8: Using the Utilities

Chapter 9: Networking

Chapter 10: Maintenance

Cleaning Radioactive or Biohazardous Materials out of the Block

Special procedures for decontaminating the block, consulting safety officers.

Changing the Fuses

Step-by-step guide for replacing blown fuses safely.

Chapter 11: Troubleshooting

Error Messages

Lists and explains common error messages and their causes/actions.

Problems Related to Protocols

Addresses issues arising from protocol setup or reaction components.

Problems Related to Environmental Conditions, Setup, and Maintenance

Solutions for issues linked to environment, installation, or upkeep.

Appendix A: Warranty Information

Exclusions from warranty

Lists conditions and actions that void the warranty.

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