BIO RAD S3e - Lasers and Filter-Sets

6
Lasers/Filter-Sets
Laser
Filter
Emission
Filter
Fluorophore or
Dye
Fluorophore
Excitation Maximum
Fluorophore
Emission Maximum
488
FL1
BP 525/30
EGFP/GFP
FITC
Alexa Fluor 488
EYFP/YFP
488
495
499
514
507
519
519
527
FL2
BP 586/25
PE
RFP
496, 565
554
578
584
FL3
BP 615/25
PI
PE-Texas Red
536
496, 565
617
619
FL4
655/LP
PE-Cy5
PE-Cy5.5
PE-Cy7
496, 565
490, 565
496, 565
670
693
785
561
FL2
BP 586/25
PE
tdTomato
RFP
DsRed
496, 565
554
554
554
578
581
584
586
FL3
BP 615/25
mCherry
PI
PE-Texas Red
587
536
496, 565
610
617
619
FL4
655/LP
PE-Alexa Fluor 647
PE-Cy5
PE-Cy5.5
PE-Cy7
496, 565
496, 565
496, 565
496, 565
669
670
693
785
BP stands for bandpass filter
LP stands for longpass filter