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Trans-Blot SD
BIO RAD Trans-Blot SD - User Manual
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T
rans-Blot
®
SD
Semi-Dry
Electr
ophor
etic
T
ransfer Cell
Instruction
Manual
Catalog Number
170-3940
For Technical Service Call Your Local Bio-Rad Office or in the U.S. Call
1-800-4BIORAD
(1-800-424-6723)
2
Table of Contents
Main Page
Default Chapter
3
Table of Contents
3
Introduction
4
Specifications
4
Section 1 Introduction
4
Equipment and Reagents
5
Equipment and Accessories
5
Related Instruments
7
Chemical Reagents
7
Safety Instructions
8
Section 3 Safety Instructions
8
Trans-Blot SD Assembly
9
Preparation for Blotting
9
Assembly of the Unit for Standard Transfers
10
Assembly of the Unit for Acidic Transfers
13
Buffer Formulation
13
Examples of Specific Protocols
14
SDS-Protein Blotting
14
DNA Blotting (for Acrylamide Gels with DNA 250 Bp to ~1 Kb)
15
DNA & RNA Blotting (for Agarose Gels with DNA up to 23 Kb, RNA up to 3.5 Kb)
15
Properties of Protein Blotting Media
15
Troubleshooting Guide
16
Poor Transfer
16
Section 8 Troubleshooting Guide
16
Poor Binding to Nitrocellulose Membrane
17
High Background after Incubation with Antibody Probes; Nonspecific or Nonquantitative Detection
17
Poor Detection Sensitivity or no Reactivity
18
References
18
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BIO RAD Trans-Blot SD Specifications
General
Brand
BIO RAD
Model
Trans-Blot SD
Category
Laboratory Equipment
Language
English
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