Table of Contents
Page
Section 1 Introduction.................................................................................................. 1
1.1 Specifications ............................................................................................................. 1
Section 2 Equipment and Reagents............................................................................ 2
2.1 Equipment and Accessories....................................................................................... 2
2.2 Related Instruments.................................................................................................... 4
2.3 Chemical Reagents..................................................................................................... 4
Section 3 Safety Instructions ....................................................................................... 5
Section 4 Trans-Blot SD Assembly ............................................................................. 6
4.1 Preparation for Blotting ............................................................................................. 6
4.2 Assembly of the Unit for Standard Transfers............................................................ 7
4.3 Assembly of the Unit for Acidic Transfers ............................................................... 10
Section 5 Buffer Formulation...................................................................................... 10
Section 6 Examples of Specific Protocols................................................................... 11
6.1 SDS-Protein Blotting ................................................................................................. 11
6.2 DNA Blotting (For acrylamide gels with DNA 250 bp to ~1 kb) ............................ 12
6.3 DNA & RNA Blotting (For agarose gels with DNA up to 23 kb,
RNA up to 3.5 kb)...................................................................................................... 12
Section 7 Properties of Protein Blotting Media......................................................... 12
Section 8 Troubleshooting Guide................................................................................ 13
8.1 Poor Transfer.............................................................................................................. 13
8.2 Poor Binding to Nitrocellulose Membrane................................................................ 14
8.3 High Background After Incubation with Antibody Probes; Nonspecific
or Nonquantitative Detection..................................................................................... 14
8.4 Poor Detection Sensitivity or No Reactivity ............................................................. 15
Section 9 References..................................................................................................... 15
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