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• Operate quickly when performing the “hot swap” procedure, as time is limited
to three minutes for the operation. Bring containers of water or sheath fluid in advance.
• The maximum total volumes for a 5 and 1.5 ml collection tubes are 4 and 1 ml,
respectively. Going above these will cause spilling and risk contamination
• You must empty the waste and refill the fluidic containers at the end of the use
• Perform a high-pressure cleaning protocols if you are the last user of the day.
Otherwise, perform a low-pressure cleaning procedure
• Use only 70% ethanol and MiliQ water as cleaning solutions
• Please let us know if some solution or fluidic is running out
• Make sure to save all the data to your own storage device or cloud as we
routinely wipe out the computer’s data
• Do not forget to register your utilization in the logbook. Remember that future
maintenance costs and repairs will be supported by the groups according to the
logbook data
• This equipment is only available for booking in the ITQB intranet
Available Techniques
• Fluorescence-Activated Cell Sorting (FACS)
• Flow Cytometry Analysis
Set Up
Sample Preparation
Cells Suspension
To avoid clogs when working with cells, resuspend cells into a single cell suspension
and always use a 40 µm or smaller filter prior to cell sorting
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