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biochrom NanoVue Plus - Default Chapter; Table of Contents

biochrom NanoVue Plus
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2
5061-049 REV 1.4
25-9574-75UM AE 05/2010
2
1. INSTALLATION 3
1.1. Unpacking and positioning 3
1.2. Safety 3
4
2. INTRODUCTION 5
2.1. Your NanoVue Plus 5
2.2. File system 6
2.3. Data export 6
2.4. Sample treatment 7
2.5. Pathlength and Absorbance nomalization 7
2.6. Auto-Read 8
2.7. Quality Assurance 8
2.8. Keypad and display 9
2.9. Software style 10
3. OPERATION AND MAINTENANCE 11
3.1. Sample application guide 11
3.2. Sample Plate replacement 12
3.3. Cleaning and general maintenance 13
3.4. Return for repair 13
3.5. Lamp replacement 13
3.6. Replacing the printer paper 13
4. LIFE SCIENCE 14
4.1. File system 14
4.2. Nucleic acids 14
4.2.1. Theory 14
4.2.2. DNA measurement 16
4.2.3. RNA measurement 17
4.2.4. Oligonucleotide measurement 19
4.2.5. T
m
Calculation 20
4.2.6. CyDye™ measurement 23
4.3. Proteins 26
4.3.1. Theory 26
4.3.2. Protein UV 28
4.3.3. Protein A280 30
4.3.4. BCA 31
4.3.5. Bradford 34
4.3.6. Lowry 36
4.3.7. Biuret 39
5. APPLICATIONS 42
5.1. File system 42
5.2. Single Wavelength 42
5.3. Concentration 44
5.4. Wavescan 46
5.5. Kinetics 48
5.6. Standard Curve 50
5.7. Multiple Wavelength 53
5.8. Absorbance Ratio 54
6. FAVOURITES AND METHODS 56
7. UTILITIES 57
7.1. Date and time 57
7.2. Regional 57
7.3. Printer 58
7.4. Preferences 58
7.5. Contrast 58
7.6. About 58
7.7. Pathlength Check & Calibration 59
7.8. Games 62
8. ACCESSORIES 63
8.1. Printer installation 63
8.2. Bluetooth accessory installation 65
8.3. Fitting SD memory card accessory 67
8.4. After sales support 70
9. TROUBLESHOOTING 71
9.1. Pathlength calibration over-range 71
9.2. Error messages 72
9.3. Fault analysis 74
9.4. Frequently asked questions 74
10. SPECIFICATION AND WARRANTY 77
11. LEGAL
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